Powered by Bioz
Troubleshooting PCR Part Two: What to Do When You Are Not Getting Bands
PCR (Polymerase Chain Reaction), which is so common to the lab, can be very frustrating. We unders...
Bovine Serum Albumin (BSA), Fraction V, Fatty Acid Free for tissue culture
Product Description
GoldBio's Reagent Grade, Fatty Acid Free BSA is ideally suited for fatty acid sensitive cell culture systems, such as CHO, Vero and MDCK cells, as well as for the most sensitive diagnostic and research applications.
GoldBio BSA is a highly purified, lyophilized albumin, suitable for Westerns, enzyme systems, protein standards, hybridizations and as a protease-sensitive immunoassays. It is also perfectly suitable as a nutrient in cell culture media, with low interference and backgrounds and is ideal for most standard molecular biology experiments. BSA protein is a single polypeptide chain consisting of ~583 amino acid residues and no carbohydrates and is approximately 66 kDa in size. Fraction V refers to the fifth fraction of the original Cohn purification of plasma proteins using cold ethanol precipitation and is still commonly used as a name for bovine serum albumin regardless of purification method.
GoldBio BSA is protease free and certified TSE free, manufactured in the United States in a closed loop system from USDA-inspected, healthy animals located in the US.
REAGENT GRADE / FATTY ACID FREE / PROTEASE FREE / IgG FREE
Applications
- Protease-sensitive Immunoassays such as RIA, EIA, Fluorescent and Chemiluminescent
- Protein Standard, Diluent
- Protein, Conjugate or Enzyme Stabilizer
- Hybridization, nucleic acid-based assays Fatty acid sensitive cell culture systems
Specifications
|
Protein (on Dried Basis) |
≥98.0% |
|
Purity (albumin) |
≥98.0% |
|
Moisture |
≤5.0% |
|
pH (10% in Water) |
6.5 to 7.5 |
|
Ash (Residue on Ignition) |
<2.0% |
|
Protease (Enzymatic Assay) |
None Detected |
|
Non-Esterified Fatty Acids (NEFA) |
<0.01 Units/mg |
|
Endotoxin |
≤1 EU/mg |
|
Heavy Metals (Pb) |
≤10 ppm |
|
IgG |
None Detected |
Storage/Handling
Store at 4°C.
Product Specifications
| Name(s) |
Albumin Bovine Serum, Fraction V; BSA, Bovine Albumin |
|---|---|
| Catalog ID | A-421 |
| CAS # | 9048-46-8 |
| MW | ~66 kDa |
| Grade | REAGENT GRADE |
| Purity | ≥98.0% |
| Storage/Handling | Store at 4°C. |
Technical Documentation
P
Protocol
O
Other
C
Certificate of Analysis
S
Safety Data Sheet
Citations
Featured Articles
What BSA (Bovine Serum Albumin) Should I Use – Your BSA Selection Guide
Bovine Serum Albumin (BSA) comes in many forms, which is why people frequently post questions onli...
6 Important FAQs About Bovine Serum Albumin (BSA)
Bovine Serum Albumin (BSA) is universal in the lab, having use in Western blot, cell tissue cultur...
