Ampicillin (Sodium) 100 mg/mL Solution
Ampicillin sodium is a member of the extended-spectrum β-lactam family and similar in structure to penicillin. It is used in the selection of antibiotic cell hybrids and has a high rate of action against many gram-negative bacterial strains. Ampicillin sodium targets non-ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. It has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus species. Resistance to ampicillin is routinely utilized as a selectable marker to confirm successful cell transformation. Ampicillin Sodium is freely soluble in water.
β-lactams inhibit the formation of peptidoglycan cross-links within bacterial cell walls by targeting penicillin-binding proteins or PBPs. Consequently, the bacterial cell wall becomes weak and cytolysis occurs. Resistance to β-lactam antibiotics occurs in the presence of cells containing plasmid encoded extended spectrum β-lactamases or ESBLs.
GoldBio’s antibiotic solutions are provided ready to use.
Sodium Ampicillin in H2O
(100 mg/mL Premade Solution, 0.2 µm Sterile Filtered)
MOLECULAR BIOLOGY GRADE
MW: 371.39 g/mol
Storage/Handling: Store at -20°C
PubChem Chemical ID: 23663979
|Grade||MOLECULAR BIOLOGY GRADE|
A brief explanation of the disk diffusion assay method used to test Gold Bio antibiotics
Procedure on the use of different antibiotics to eliminate fungal, bacterial and mycoplasma contamination of cell lines
Sample Certificate of Analysis - Ampicillin
COA for example only. Actual tests may differ.