EHA105 (pSoup-P19) Agrobacterium Chemically Competent Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-128-5x50 | 5 x 50 μL |
|
$ 161.00 |
CC-128-10x50 | 10 x 50 μL |
|
$ 285.00 |
CC-128-15x50 | 15 x 50 μL |
|
$ 382.00 |
Description
GoldBio’s EHA105 (pSoup-P19) chemically competent Agrobacterium cells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, chloramphenicol, and streptomycin, providing versatility in selection and antibiotic resistance EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene.
The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids, increasing the range of applications for the EHA105 (pSoup) system. The p19 protein is derived from tomato bush dwarf virus and further improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign. These competent cells are suitable for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.
GoldBio’s EHA105 Agrobacteriumstrain was generated, and primary clone supplied by Dr. Elizabeth Hood.
Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: EHA105 (pSoup-P19)
Transformation efficiency: ≥2 x 103 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
EHA105 (pSoup-P19) Agrobacterium ElectroCompetent Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-245-5x50 | 5 x 50 μL |
|
$ 170.00 |
CC-245-10x50 | 10 x 50 μL |
|
$ 306.00 |
CC-245-15x50 | 15 x 50 μL |
|
$ 413.00 |
Description
EHA105 (pSoup-P19) ElectroCompetent Agrobacterium cells are designed to provide high transformation efficiencies for applications such as cDNA or gDNA library construction. The strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA), which carries the vir gene necessary for the insertion of T-DNA into the plant genome. Although the pEHA105 plasmid is incapable of transferring its own T-DNA, it can still transfer foreign binary vector T-DNA.
The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and further improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign. These features help to enhance the Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.
GoldBio’s EHA105 Agrobacterium strain was generated, and primary clone supplied by Dr. Elizabeth Hood.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: EHA105 (pSoup-P19)
Transformation efficiency: ≥3 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
EHA105 Agrobacterium Chemically Competent Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-108-5x50 | 5 x 50 μL |
|
$ 161.00 |
CC-108-10x50 | 10 x 50 μL |
|
$ 285.00 |
CC-108-15x50 | 15 x 50 μL |
|
$ 382.00 |
Description
GoldBio’s EHA105 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene. EHA105 is ideal for genetic transformation of rice, tobacco and several other plants.
Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: EHA105
Transformation efficiency: ≥5 x 104 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
EHA105 Agrobacterium ElectroCompetent Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-225-5x50 | 5 x 50 μL |
|
$ 170.00 |
CC-225-10x50 | 10 x 50 μL |
|
$ 306.00 |
CC-225-15x50 | 15 x 50 μL |
|
$ 413.00 |
Description
GoldBio’s EHA105 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene. EHA105 is ideal for genetic transformation of rice, tobacco and several other plants.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: EHA105
Transformation efficiency: ≥1.6 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GV3101 (pSoup) Agrobacterium Chemically Competent Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-115-5x50 | 5 x 50 μL |
|
$ 161.00 |
CC-115-10x50 | 10 x 50 μL |
|
$ 285.00 |
CC-115-15x50 | 15 x 50 μL |
|
$ 382.00 |
Description
GoldBio’s GV3101 Agrobacterium chemically competent cells are a powerful tool for high efficiency transformation of plants. The GV3101 strain is optimized for cDNA and gDNA library construction, and possesses resistance to rifampicin, gentamicin, and tetracycline. The T-DNA region of the GV3101 Ti plasmid has been deleted, but restoration of the binary vector T-region can fully enable T-DNA binary system function.
The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids, and also helps with Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.
Product Specifications
Competent cell type: Chemically Competent
Species: A. tumefaciens
Strain: GV3101 (pSoup)
Transformation efficiency: ≥1.5 x 103 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GV3101 (pSoup) Agrobacterium Electrocompetent Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-217-5x50 | 5 x 50 μL |
|
$ 170.00 |
CC-217-10x50 | 10 x 50 μL |
|
$ 306.00 |
CC-217-15x50 | 15 x 50 μL |
|
$ 413.00 |
Description
GoldBio’s GV3101 Agrobacterium electrocompetent cells are a powerful tool for high efficiency transformation of plants. The GV3101 strain is optimized for cDNA and gDNA library construction, and possesses resistance to rifampicin, gentamicin, and tetracycline. The T-DNA region of the GV3101 Ti plasmid has been deleted, but restoration of the binary vector T-region can fully enable T-DNA binary system function.
The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids, and also helps with Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: GV3101 (pSoup)
Transformation efficiency: ≥8 x 107 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GV3101 (pSoup-P19) Agrobacterium Chemically Competent Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-125-5x50 | 5 x 50 μL |
|
$ 161.00 |
CC-125-10x50 | 10 x 50 μL |
|
$ 285.00 |
CC-125-15x50 | 15 x 50 μL |
|
$ 382.00 |
Description
GoldBio’s GV3101 competent Agrobacterium cells are a highly efficient tool for genetic transformation of dicotyledonous and monocotyledonous plant species, such as Arabidopsis thaliana, tobacco, potato, and corn. These cells carry the nopaline-type Ti plasmid pMP90 (pTiC58DT-DNA) that contains the vir genes required for the transfer and integration of T-DNA into the plant genome. Additionally, the cells are resistant to rifampicin, gentamicin, and tetracycline, which allows for selection of transformed cells.
The pSoup plasmid carried by the cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and further improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign genes. These combined properties make GV3101 competent Agrobacterium cells an ideal choice for cDNA or gDNA library construction and the generation of transgenic plants with desirable traits.
Product Specifications
Competent cell type: Chemically Competent
Species: A. tumefaciens
Strain: GV3101
(pSoup-P19)
Transformation efficiency: ≥3 x 103 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GV3101 (pSoup-P19) Agrobacterium Electrocompetent Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-227-5x50 | 5 x 50 μL |
|
$ 170.00 |
CC-227-10x50 | 10 x 50 μL |
|
$ 306.00 |
CC-227-15x50 | 15 x 50 μL |
|
$ 413.00 |
Description
GoldBio’s GV3101 electrocompetent Agrobacterium cells are a highly efficient tool for genetic transformation of dicotyledonous and monocotyledonous plant species, such as Arabidopsis thaliana, tobacco, potato, and corn. These cells carry the nopaline-type Ti plasmid pMP90 (pTiC58DT-DNA) that contains the vir genes required for the transfer and integration of T-DNA into the plant genome. Additionally, the cells are resistant to rifampicin, gentamicin, and tetracycline, which allows for selection of transformed cells.
The pSoup plasmid carried by the cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign genes. These combined properties make GV3101 electrocompetent Agrobacterium cells an ideal choice for cDNA or gDNA library construction and the generation of transgenic plants with desirable traits.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: GV3101 (pSoup-P19)
Transformation efficiency: ≥1 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GV3101 Agrobacterium Chemically Competent Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-105-5x50 | 5 x 50 μL |
|
$ 161.00 |
CC-105-10x50 | 10 x 50 μL |
|
$ 285.00 |
CC-105-15x50 | 15 x 50 μL |
|
$ 382.00 |
Description
GoldBio’s GV3101 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our GV3101 strain harbors the C58 chromosomal backbone containing rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) harboring the gentamicin resistance. A functional T-DNA binary system can be built using our GV3101 strains as the T-DNA region has been deleted in the Ti plasmid and instead has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, tobacco, potato, soybeans and corn.
Product Specifications
Competent cell type: Chemically Competent
Species: A. tumefaciens
Strain: GV3101
Transformation efficiency: ≥6 x 104 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GV3101 Agrobacterium Electrocompetent Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-207-5x50 | 5 x 50 μL |
|
$ 170.00 |
CC-207-10x50 | 10 x 50 μL |
|
$ 306.00 |
CC-207-15x50 | 15 x 50 μL |
|
$ 413.00 |
Description
GoldBio’s GV3101 Agrobacterium Electrocompetent Cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our GV3101 strain harbors the C58 chromosomal backbone containing rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) harboring the gentamicin resistance. A functional T-DNA binary system can be built using our GV3101 strains as the T-DNA region has been deleted from the Ti plasmid and instead has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, tobacco, potato, soybeans and corn.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: GV3101
Transformation efficiency: ≥2 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
HB101 Chemically Competent E. coli Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-150-5x50 | 5 x 50 µl |
|
$ 51.00 |
CC-150-10x50 | 10 x 50 µl |
|
$ 90.00 |
Description
GoldBio’s HB101 chemically competent E. coli cells allow you to obtain high transformation efficiency in applications such cloning and sub-cloning. Our E. coli HB101 proceeds from the K12 x B hybrid strain. HB101 strain also contains the recA13 mutation useful in the insert stability and minimization of recombination. E. coli HB101 also has the hsdS20(rB-mB-) genotype that prevents the cleavage of cloned DNA by endogenous restriction enzymes.
Product Specifications
Competent cell type: Chemically competent
Derivative of: HB101
Species: E. coli
Transformation efficiency: ≥7 x 106 cfu/µg pUC19 DNA
Blue/white screening: No
IPTG
Catalog ID | Size | Pricing | |
---|---|---|---|
I2481C | 1 g |
|
$ 22.00 |
I2481C5 | 5 g |
|
$ 30.00 |
I2481C25 | 25 g |
|
$ 124.00 |
I2481C50 | 50 g |
|
$ 192.00 |
I2481C100 | 100 g |
|
$ 353.00 |
I2481C200 | 200 g |
|
$ 676.00 |
I2481C300 | 300 g |
|
$ 901.00 |
I2481C400 | 400 g |
|
$ 1,169.00 |
I2481C500 | 500 g |
|
$ 1,407.00 |
Description
IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.
Product Specifications
Catalog ID | I2481 |
---|---|
Name(s) |
IPTG Isopropyl-beta-D-thiogalactoside |
CAS # | 367-93-1 |
Formula | C9H18O5S |
MW | 238.30 g/mol |
Grade | MOLECULAR BIOLOGY GRADE |
Storage/Handling | Store desiccated at -20°C. Protect from light. |
JM109 Chemically Competent E. coli Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-114-5x50 | 5 x 50 µl |
|
$ 51.00 |
CC-114-10x50 | 10 x 50 µl |
|
$ 90.00 |
Description
GoldBio’s JM109 Chemically Competent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, library construction, cloning, and subcloning. JM109 is a K strain bacterium that carries multiple features including the ɸ80lacZ∆M15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol as well as the hsdR17 genotype which prevents endogenous endonucleases from cleaving DNA.
Product Specifications
Competent cell type: Chemically competent
Derivative of: JM109
Species: E. coli
Format: Tubes
Transformation efficiency: ≥6 x 105 cfu/µg pUC19 DNA
Blue/white screening: Yes
Product Specifications
Catalog ID | CC-114 |
---|---|
Storage/Handling | Store Competent Cells at -80°C. |
LBA4404 Agrobacterium Chemically Competent Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-107-5x50 | 5 x 50 μL |
|
$ 161.00 |
CC-107-10x50 | 10 x 50 μL |
|
$ 285.00 |
CC-107-15x50 | 15 x 50 μL |
|
$ 382.00 |
Description
GoldBio’s LBA4404 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbors a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octoprine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes. Our LBA4404 strain can be used in genetic transformation of tomato, tobacco and other plants.
Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: LBA4404
Transformation efficiency: ≥1 x 104 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
LBA4404 Agrobacterium ElectroCompetent Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-220-5x50 | 5 x 50 μL |
|
$ 170.00 |
CC-220-10x50 | 10 x 50 μL |
|
$ 306.00 |
CC-220-15x50 | 15 x 50 μL |
|
$ 413.00 |
Description
GoldBio’s LBA4404 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbor a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octoprine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes. Our LBA4404 strain can be used in genetic transformation of tomato, tobacco and other plants.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: LBA4404
Transformation efficiency: ≥4 x 106 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
RR1 Chemically Competent E. coli Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-113-5x50 | 5 x 50 µl |
|
$ 51.00 |
CC-113-10x50 | 10 x 50 µl |
|
$ 90.00 |
Description
GoldBio’s RR1 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli RR1 is a recA+ derivative of the HB101 strain and can be more useful than HB101 if a recA+ background is required.
Product Specifications
Competent cell type: Chemically competent
Derivative of: RR1
Species: E. coli
Format: Tubes
Transformation efficiency: ≥3 x 107 cfu/µg pUC19 DNA
Blue/white screening: No
Product Specifications
Catalog ID | CC-113 |
---|---|
Storage/Handling | Store Competent Cells at -80°C. |
TG1 Phage Display Electrocompetent Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-205-5x100 | 5 x 100 μL |
|
$ 160.00 |
CC-205-10x100 | 10 x 100 μL |
|
$ 278.00 |
CC-205-15x100 | 15 x 100 μL |
|
$ 405.00 |
Description
GoldBio’s TG1 Phage Display Electrocompetent E. coli cells are suitable for protein expression and preparation of antibody or peptide phage display libraries.TG1 Phage Display cells have ≥1 x 109 cfu/µg efficiency with electroporation and have Amber suppressor strain (supE).
Product Specifications
Competent cell type: ElectroCompetent
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 109 cfu/µg pUC19 DNA
Blue/white screening: Yes