Protein A
Gold Biotechnology offers Protein A agarose resins for antibody purification. Protein A is a cell wall component of Staphylococcus aureus and consists of a cylindrical, single polypeptide chain that contains 5 antibody-binding domains. These high affinity regions specifically bind to Fc regions of immunoglobulins of various species. Protein A resins have been widely used to purify immunoglobulins of different mammalian species and also IgG subclasses, which have no specific affinity. Our Protein A resins are temperature stable, retain their native conformation even in the presence of denaturing agents, have a high IgG-binding capacity and binding stability and benefit from milder elution conditions than Protein G agarose resin. Protein A is ideally recommended for applications involving various human IgG (excluding IgG3) and mouse IgG2 as well as IgG from pigs, guinea pigs and rabbits.
Protein A Agarose Resin (Pharmaceutical grade for research)
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| P-400-5 | 5 mL | $ 201.00 | |
| P-400-25 | 25 mL | $ 778.00 | |
| P-400-50 | 50 mL | $ 1,427.00 | |
| P-400-100 | 100 mL | $ 2,420.00 |
Description
Protein A agarose resin consists of rProtein A covalently bound to cross-linked agarose beads. It provides a very stable bond that can greatly minimize leakage of the Protein A allowing for reuse of the affinity resin in several purification steps. Protein A Agarose resin is available in bulk from 5-100 ml quantities.
Protein A resin is good for the purification of a wide range of Immunoglobulins of different mammalian species and also certain IgG subclasses that have no other affinity (see additional information).
Protein A is a cell wall component of Staphylococcus aureus. It consists of a single polypeptide chain shaped as a cylinder, which contains five antibody–binding domains. These high affinity regions are specifically bonded to Fc region of the immunoglobulins (specially IgGs of different species).
Product Specifications
| Catalog ID | P-400 |
|---|---|
| Storage/Handling | store at 4°C. Do NOT freeze. |
Protein A Magnetic Agarose Beads
Protein A Magnetic Agarose Beads
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| P-404-2 | 2 mL | $ 124.00 | |
| P-404-5 | 5 mL | $ 293.00 |
Description
Protein A Magnetic Agarose Beads are designed to provide the easiest
and simplest method of monoclonal and polyclonal antibody purification.
The antibodies are retained to the magnetic resin which are also
covalently conjugated with recombinant Protein A. Magnetic agarose beads
provide a convenient and quick method for purification without the need
for pipetting or centrifugation. Washing, binding and elution steps
will require a magnetic device.
Protein A Magnetic Beads are supplied as a 50% suspension of magnetic beads in 20% ethanol (Each 1000 μl of suspension contains 500 μl of magnetic beads.)
Binding Capacity: ~10 mg human IgG / ml resin.
Product Specifications
| Catalog ID | P-404 |
|---|---|
| Storage/Handling | store at 4°C. Do NOT freeze. |
