G_Products
N-gamma-Hydroxy-L-arginine, acetate salt
Catalog ID | Size | Pricing | |
---|---|---|---|
H-360-25 | 25 mg | $ 129.00 | |
H-360-50 | 50 mg | $ 240.00 | |
H-360-100 | 100 mg | $ 452.00 | |
H-360-250 | 250 mg | $ 1,065.00 | |
H-360-500 | 500 mg | $ 1,826.00 |
Description
N-gamma-Hydroxy-L-arginine is an intermediate in the biosynthesis of nitric oxide from L-arginine. Arginase is inhibited by N-gamma-Hydroxy-L-arginine, as is the cell proliferation of MDA-MB-468 cancer cells.
Product Specifications
Catalog ID | H-360 |
---|---|
CAS # | 53598-01-9 |
MW | 250.25 g/mol |
ProBlock™ Gold 2D Protease Inhibitor Cocktail (EDTA Free) [100X]
Catalog ID | Size | Pricing | |
---|---|---|---|
GB-109-1 | 1 mL | $ 205.00 |
Description
ProBlock™ Gold 2D is a protease inhibitor cocktail specifically developed for sample preparation for IEF/2D- studies. No EDTA is used, which allows optimal action of nuclease activity for removing nucleic acids from the samples.
ProBlock™ Gold 2D contains both irreversible and reversible protease inhibitors and inhibits serine, cysteine and metalloproteases etc. Due to the optimized concentration of the various inhibitors, the ProBlock™ Gold 2D shows excellent inhibition of protease activities and is therefore suitable for the protection of proteins extracted from animal cells / tissues, plants, yeast and bacteria etc.
ProBlock™ Gold 2D at 1X concentration in extraction buffer at pH 7-8 inhibits over 90% of protease activities.
Product Specifications
Catalog ID | GB-109 |
---|---|
Storage/Handling | Store at -20°C. |
ProBlock™ Gold Bacterial 2D Protease Inhibitor Cocktail [100X]
Catalog ID | Size | Pricing | |
---|---|---|---|
GB-376-1 | 1 mL | $ 79.00 | |
GB-376-5 | 5 mL | $ 299.00 |
Description
A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail. ProBlock™ Gold Bacterial 2D contains optimized concentrations of various bacterial protease inhibitors, which provide excellent inhibition of protease activities during recombinant protein purification from bacteria. The cocktail contains both reversible and irreversible protease inhibitors to inhibit metallo, serine and cysteine proteases. It also contains specific inhibitors for bacterial proteases, such as aspartic proteases and aminopeptidases. A 1X concentration of Bacterial ProteaseArrest™ inhibits over 90% of protease activities.
ProBlock™ Gold Bacterial 2D cocktail does not use EDTA as its metalloprotease inhibitor as it would inhibit the activity of proteins that require divalent cations (Ca2+, Mg2+, or Mn2+) for their biological activity. In addition, EDTA would inhibit the purification of proteins using immobilized metal affinity chromatography (IMAC), for example His tagged or CBP tagged proteins. ProBlock™ Gold Bacterial 2Dcocktail is compatible with immobilized metal affinity chromatography.
Product Specifications
Catalog ID | GB-376 |
---|---|
Storage/Handling | Store at 4°C. |
ProBlock™ Gold Bacterial Protease Inhibitor Cocktail [100X]
Catalog ID | Size | Pricing | |
---|---|---|---|
GB-330-1 | 1 mL | $ 85.00 | |
GB-330-5 | 5 mL | $ 295.00 |
Description
ProBlock™ Gold Bacterial Protease Inhibitor Cocktail contains optimized concentrations of various bacterial protease inhibitors, which provide excellent inhibition of protease activities during protein purification from bacteria. The cocktail contains both reversible and irreversible protease inhibitors to inhibit serine and cysteine proteases. It also contains specific inhibitors for bacterial proteases, such as aspartic proteases and aminopeptidases. A 1X concentration of ProBlock™ Gold Bacterial inhibits over 90% of protease activities.
Some proteins require divalent cations (Ca2+, Mg2+ or Mn2+) for their biological activity and the presence of chelating agents may be detrimental to the protein’s activity. Chelators, such as EDTA, inhibit the purification of proteins by immobilized metal affinity chromatography.
ProBlock™ Gold Bacterial is supplied with additional, optional EDTA and 1,10-Phenanthroline monohydrate which are provided for optimal inhibition of metalloproteases and may be added in the extraction buffer or lysate as needed.
Product Specifications
Catalog ID | GB-330 |
---|---|
Storage/Handling | Store at 4°C. |
ProBlock™ Gold Extra Strength Protease Inhibitor Cocktail
Catalog ID | Size | Pricing | |
---|---|---|---|
GB-116-2 | 2 mL | $ 175.00 | |
GB-116-5 | 5 mL | $ 345.00 | |
GB-116-10 | 10 mL | $ 605.00 |
Description
GoldBio’s new ProBlock™ Gold Extra Strength Protease Inhibitor Cocktail is specifically formulated for the most demanding protein purification applications. The optimized concentrations of the various inhibitors in our Extra Strength cocktail deliver an enhanced dose of protease inhibition for the toughest research experiments. ProBlock™ Gold Extra Strength contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases.
Product Specifications
Catalog ID | GB-116 |
---|---|
Storage/Handling | Store at 4°C. |
ProBlock™ Gold Mammalian Protease Inhibitor Cocktail [100X]
Catalog ID | Size | Pricing | |
---|---|---|---|
GB-331-1 | 1 mL | $ 85.00 | |
GB-331-5 | 5 mL | $ 295.00 |
Description
ProBlock™ Gold mammalian protease inhibitor cocktail contains optimized concentrations of various mammalian protease inhibitors, which provide excellent inhibition of protease activities during protein purification from mammalian cells and tissues. The cocktail contains both reversible and irreversible protease inhibitors to inhibit serine, cysteine and specific inhibitors for mammalian proteases, such as aminopeptidases. A 1X concentration of ProBlock™ Gold Mammalian inhibits over 90% of protease activities.
Some proteins require divalent cations (Ca2+, Mg2+ or Mn2+) for their biological activity and the presence of chelating agents may be detrimental to the protein’s activity. Chelators, such as EDTA, inhibit the purification of proteins by immobilized metal affinity chromatography.
ProBlock™ Gold Mammalian is supplied with additional, optional EDTA and 1,10-Phenanthroline monohydrate which are provided for optimal inhibition of metalloproteases and may be added in the extraction buffer or lysate as needed.
Product Specifications
Catalog ID | GB-331 |
---|---|
Storage/Handling | Store at 4°C. |
ProBlock™ Gold Plant Protease Inhibitor Cocktail [100X]
Catalog ID | Size | Pricing | |
---|---|---|---|
GB-332-1 | 1 mL | $ 79.00 | |
GB-332-5 | 5 mL | $ 289.00 |
Description
ProBlock™ Gold Plant protease inhibitor cocktail contains optimized concentrations of various plant protease inhibitors, which provide excellent inhibition of protease activities during protein purification from plant. The cocktail contains both reversible and irreversible protease inhibitors to inhibit serine, cysteine, metalloproteases and specific inhibitors for plant proteases, such as aspartic proteases and aminopeptidases. A 1X concentration oft ProBlock™ Gold Plant inhibits over 90% of protease activities.
Product Specifications
Catalog ID | GB-332 |
---|---|
Storage/Handling | Store at 4°C. |
ProBlock™ Gold Protease Inhibitor Cocktail [100X]
Catalog ID | Size | Pricing | |
---|---|---|---|
GB-108-2 | 2 mL | $ 125.00 | |
GB-108-5 | 5 mL | $ 255.00 | |
GB-108-10 | 10 mL | $ 449.00 |
Description
Due to the optimized concentration of the various inhibitors, the ProBlock™ Gold shows excellent inhibition of protease activities and is therefore suitable for the protection of proteins during purification from animal tissues, plant tissues, yeast and bacteria. ProBlock™ Gold contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. An separate bottle of EDTA is provided to inhibit metalloproteases.
Since some proteins require divalent cations like Ca2+, Mg2+ or Mn2+ for their biological activity, the presence of EDTA may be detrimental to the protein activity. Furthermore, if the protein of interest is purified using immobilized metal chelate affinity chromatography, EDTA must be removed from the buffer before the chromatography. The ProBlock™ Gold is therefore supplied with a separate, optional EDTA solution which may be added to the extraction buffer or lysate as needed.
Product Specifications
Catalog ID | GB-108 |
---|---|
Storage/Handling | Store at 4°C. |
ProBlock™ Gold Yeast/Fungi Protease Inhibitor Cocktail [100X]
Catalog ID | Size | Pricing | |
---|---|---|---|
GB-333-1 | 1 mL | $ 85.00 | |
GB-333-5 | 5 mL | $ 295.00 |
Description
ProBlock™ Gold Yeast/Fungi protease inhibitor cocktail contains optimized concentrations of various yeast and fungal protease inhibitors, which provide excellent inhibition of protease activities during protein purification from yeast or fungi. The cocktail contains both reversible and irreversible protease inhibitors to inhibit serine, cysteine, metalloproteases and specific inhibitors for yeast or fungal proteases, such as aspartic proteases. At 1X concentration, ProBlock™ Gold Yeast/Fungi inhibits over 90% of protease activities.
Some proteins require divalent cations (Ca2+, Mg2+ or Mn2+) for their biological activity and the presence of chelating agents may be detrimental to the protein’s activity. Chelators, such as EDTA, inhibit the purification of proteins by immobilized metal affinity chromatography.
ProBlock™ Gold Yeast/Fungi is supplied with additional, optional EDTA and 1,10-Phenanthroline monohydrate which are provided for optimal inhibition of metalloproteases and may be added in the extraction buffer or lysate as needed.
Product Specifications
Catalog ID | GB-333 |
---|---|
Storage/Handling | Store at 4°C. |
Protein G HTC Agarose Beads
Catalog ID | Size | Pricing | |
---|---|---|---|
P-430-5 | 5 mL | $ 375.00 | |
P-430-25 | 25 mL | $ 1,306.00 |
Description
Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains of native Protein G have been removed to ensure the maximum specific IgG binding capacity.
GoldBio offers rProtein G resins to isolate and purify classes, subclasses and fragments of immunoglobulins from cell culture media and biological fluids. Rapid purification and high yield of purified immunoglobulin are obtainable by this method.
Protein G is covalently bound to highly cross-linked agarose beads, providing a very stable bond that can greatly minimize leakage of the Protein G and allowing for reuse of the affinity resin in several purification steps. This resin works well in High Flow conditions.
Product Specifications
Catalog ID | P-430 |
---|---|
Storage/Handling | store at 4°C. Do NOT freeze. |
Protein G Magnetic Agarose Beads
Catalog ID | Size | Pricing | |
---|---|---|---|
P-434-2 | 2 mL | $ 132.00 | |
P-434-5 | 5 mL | $ 300.00 |
Description
Protein G Magnetic Agarose Beads are designed to provide the easiest
and simplest method of monoclonal and polyclonal antibody purification.
The antibodies are retained to the magnetic resin which are also
covalently conjugated with recombinant Protein G. Magnetic agarose
beads provide a convenient and quick method for purification without
the need for pipetting or centrifugation. Washing, binding and elution
steps will require a magnetic device.
Protein G Magnetic Beads are supplied as a 50% suspension of magnetic beads in 20% ethanol (Each 1000 μl of suspension contains 500 μl of magnetic beads.)
Binding Capacity: ~10 mg human IgG / ml resin.
Product Specifications
Catalog ID | P-434 |
---|---|
Storage/Handling | store at 4°C. Do NOT freeze. |
Sodium D-glucuronate
Catalog ID | Size | Pricing | |
---|---|---|---|
G-140-500 | 500 g | $ 206.00 | |
G-140-1 | 1 kg | $ 365.00 |
Description
Sodium glucuronate is a sugar derived carboxylic acid involved in glucuronidation, where glucuronic acid is bonded to a molecule, which allows for transport since glucuronate is highly water-soluble. This allows for the delivery of drugs or the removal of toxins from cells. It is used to increase cell toxin removal and to increase drug or hormone delivery to cells. and is one of the precursors to vitamin C.
Product Specifications
Catalog ID | G-140 |
---|---|
CAS # | 207300-70-7 |
MW | 234.14 g/mol |
Grade | MOLECULAR BIOLOGY GRADE |
Storage/Handling | Store at room temperature. |
Y-β-D-GLC
Catalog ID | Size | Pricing | |
---|---|---|---|
I-470-250MG | 250 mg | $ 93.00 | |
I-470-5 | 5 g | $ 867.00 |
Description
Y-β-D-GLC is a chromogenic substrate for β-D-glucosidase, yielding a blue precipitate upon cleavage.
Product Specifications
Catalog ID | I-470 |
---|---|
CAS # | 487-60-5 |
MW | 295.29 g/mol |
Grade | MOLECULAR BIOLOGY GRADE |
Storage/Handling | Store at -20°C. Protect from light. |
α-D-Galactose 1-phosphate, dipotassium salt pentahydrate
Catalog ID | Size | Pricing | |
---|---|---|---|
G-105-500 | 500 mg | $ 208.00 | |
G-105-1 | 1 g | $ 328.00 | |
G-105-5 | 5 g | $ 849.00 |
Description
α-D-Galactose 1-phosphate, dipotassium salt pentahydrate is a phosphorylated sugar produced from galactose by galactokinase. It is an intermediate found in the conversion pathway of glucose and galactose.
Product Specifications
Catalog ID | G-105 |
---|---|
CAS # | 19046-60-7 |
MW | 426.39 g/mol |
Storage/Handling | Store at -20°C. |