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Welcome to Gold Biotechnology, a Saint Louis based biotech company. For over thirty years we have endeavored to provide life scientists around the world access to the best reagents for the best price.

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BLUEstain™ Protein ladder, 11-245 kDa

Catalog ID Size Pricing
P007-500 500 µL $ 120.00   $ 102.00
P007-1000 2 x 500 μL $ 235.00   $ 200.00
P007-1500 3 x 500 μL $ 345.00   $ 294.00
P007-2000 4 x 500 μL $ 445.00   $ 379.00
P007-2500 5 x 500 μL $ 540.00   $ 459.00
P007-3000 6 x 500 μL $ 625.00   $ 532.00

Description

The BLUEstain™ Protein ladder is a three-color protein standard with 12 prestained proteins covering a wide range molecular weights from ~11 to 245 kDa. Proteins are covalently coupled with a bluechromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BLUEstain™ Protein ladder, is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.

Product Specifications

Catalog ID P007
Storage/Handling Store at 4°C for 3 months. Store at -20°C for 24 months.
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P007-500 500 µL $ 120.00
$ 102.00
P007-1000 2 x 500 μL $ 235.00
$ 200.00
P007-1500 3 x 500 μL $ 345.00
$ 294.00
P007-2000 4 x 500 μL $ 445.00
$ 379.00
P007-2500 5 x 500 μL $ 540.00
$ 459.00
P007-3000 6 x 500 μL $ 625.00
$ 532.00

1 kb DNA Ladder

Catalog ID Size Pricing
D010-500 500 µL $ 49.00   $ 42.00
D010-1000 2 x 500 μL $ 95.00   $ 81.00
D010-1500 3 x 500 μL $ 135.00   $ 115.00
D010-2000 4 x 500 μL $ 169.00   $ 144.00
D010-2500 5 x 500 μL $ 189.00   $ 161.00
D010-3000 6 x 500 μL $ 215.00   $ 183.00

Description

A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 13 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 250-10,000 base pairs. The 1K and 3K bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples. The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

Product Specifications

Catalog ID D010
Storage/Handling Store at room temperature for 6 months. Store at -20°C for 24 months.
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D010-500 500 µL $ 49.00
$ 42.00
D010-1000 2 x 500 μL $ 95.00
$ 81.00
D010-1500 3 x 500 μL $ 135.00
$ 115.00
D010-2000 4 x 500 μL $ 169.00
$ 144.00
D010-2500 5 x 500 μL $ 189.00
$ 161.00
D010-3000 6 x 500 μL $ 215.00
$ 183.00

DTT (Dithiothreitol) (> 99% pure) Protease free

Catalog ID Size Pricing
DTT10 10 g $ 59.00   $ 51.00
DTT25 25 g $ 129.00   $ 110.00
DTT50 50 g $ 229.00   $ 195.00
DTT100 100 g $ 369.00   $ 314.00
DTT500 500 g $ 1,645.00   $ 1,399.00

Description

Dithiothreitol (DTT) is the common name of the more popular of the two Cleland’s Reagents (the other being Dithioerythritol or DTE). DTT is a powerful reducing agent that forms a stable six-membered ring with an internal disulfide bond which is resistant to oxidation. DTT is often used for the following: reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl) cystamine to break apart the matrix of a polyacrylamide gel, the reduction of disulfide bonds in proteins, the prevention of those bonds from forming between cysteine residues and lastly, DTT is often used to reduce thiolated DNA in order to minimize dimerization.

DTT is a nearly 7-fold stronger reduction agent than βME (β-mercaptoethanol) and has a less offensive odor and is less toxic.

Product Specifications

Catalog ID DTT
CAS # 27565-41-9 / 3483-12-3
MW 154.25 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C.
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DTT10 10 g $ 59.00
$ 51.00
DTT25 25 g $ 129.00
$ 110.00
DTT50 50 g $ 229.00
$ 195.00
DTT100 100 g $ 369.00
$ 314.00
DTT500 500 g $ 1,645.00
$ 1,399.00

IPTG

Catalog ID Size Pricing
I2481C 1 g $ 25.00
I2481C5 5 g $ 35.00
I2481C25 25 g $ 145.00
I2481C50 50 g $ 225.00
I2481C100 100 g $ 415.00
I2481C200 200 g $ 795.00
I2481C300 300 g $ 1,059.00
I2481C400 400 g $ 1,375.00
I2481C500 500 g $ 1,655.00

Description

IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.

Product Specifications

Catalog ID I2481
Name(s) IPTG
Isopropyl-beta-D-thiogalactoside
CAS # 367-93-1
Formula C9H18O5S
MW 238.30 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
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I2481C 1 g $ 25.00
I2481C5 5 g $ 35.00
I2481C25 25 g $ 145.00
I2481C50 50 g $ 225.00
I2481C100 100 g $ 415.00
I2481C200 200 g $ 795.00
I2481C300 300 g $ 1,059.00
I2481C400 400 g $ 1,375.00
I2481C500 500 g $ 1,655.00

D-Luciferin, Potassium Salt (Proven and Published®)

Catalog ID Size Pricing
LUCK-100 100 mg $ 54.00
LUCK-250 250 mg $ 129.00
LUCK-300 300 mg $ 151.00
LUCK-500 500 mg $ 194.00
LUCK-1G 1 g $ 245.00
LUCK-2G 2 x 1 g $ 472.00
LUCK-3G 3 x 1 g $ 702.00
LUCK-4G 4 x 1 g $ 928.00
LUCK-5G 5 x 1 g $ 1,150.00
LUCK-10G 10 x 1 g $ 2,280.00

Description

Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2+ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.

Luciferin is a common reagent used throughout the biotechnology field and specifically for in vivo imaging. Luciferase labeled tumor cells, stem cells or infectious diseases are often inoculated into research animals such as rats or mice for investigation. The injection of luciferin allows for the real-time, noninvasive monitoring of disease progression and/or drug efficacy in these model systems through Bioluminescence Imaging (BLI).

Luciferin is also commonly used for in vitro research, including luciferase and ATP assays, gene reporter assays, high throughput sequencing and various contamination assays. ®

Product Specifications

Catalog ID LUCK®
CAS # 115144-35-9
MW 318.42 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
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LUCK-100 100 mg $ 54.00
LUCK-250 250 mg $ 129.00
LUCK-300 300 mg $ 151.00
LUCK-500 500 mg $ 194.00
LUCK-1G 1 g $ 245.00
LUCK-2G 2 x 1 g $ 472.00
LUCK-3G 3 x 1 g $ 702.00
LUCK-4G 4 x 1 g $ 928.00
LUCK-5G 5 x 1 g $ 1,150.00
LUCK-10G 10 x 1 g $ 2,280.00

TCEP HCl

Catalog ID Size Pricing
TCEP1 1 g $ 25.00   $ 22.00
TCEP2 2 g $ 45.00   $ 39.00
TCEP5 5 g $ 85.00   $ 73.00
TCEP10 10 g $ 135.00   $ 115.00
TCEP25 25 g $ 275.00   $ 234.00
TCEP50 50 g $ 505.00   $ 430.00
TCEP100 100 g $ 995.00   $ 846.00

Description

TCEP-HCl is an odorless reducing agent suitable for protein applications and in the preparation for electrophoresis. TCEP is water soluble, is stable in a variety of solutions and is particularly useful in the labeling of cysteine residues with maleimides, preventing the cysteines from forming disulfide bonds. As a reducing agent, TCEP has many other desirable features.

Product Specifications

Catalog ID TCEP
CAS # 51805-45-9
MW 286.65 g/mol
Storage/Handling Store desiccated at -20°C.
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TCEP1 1 g $ 25.00
$ 22.00
TCEP2 2 g $ 45.00
$ 39.00
TCEP5 5 g $ 85.00
$ 73.00
TCEP10 10 g $ 135.00
$ 115.00
TCEP25 25 g $ 275.00
$ 234.00
TCEP50 50 g $ 505.00
$ 430.00
TCEP100 100 g $ 995.00
$ 846.00

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