SuperAgro™

The pSuperAgro™ v1 Plasmid (pBBRacdS) is an Agrobacterium helper plasmid that includes 1-aminocyclopropane-1-carboxylate (ACC) deaminase (AcdS) activity, driven by the lac promoter, which suppresses ethylene evolution during co-cultivation and significantly increases T-DNA transfer.

AcdS activity is beneficial for higher callus induction frequency and also suppresses callus browning phenomena. This plasmid has been shown to be efficacious in both Hardy Pampas Grass (E. Ravennae) and Tomato plants (S. Lycopersicum).

These products are sold under license by GoldBio and require a signed agreement before fulfillment. See license details below in the technical documents.

The pSuperAgro™ v3 plasmid (pBBRgabT) was designed to include GabT activity, driven by the lac promoter, which degrades gamma-aminobutyric acid (GABA) during co-cultivation. The reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.

GabT activity decreases GABA content locally in plant cells and induces higher shoot regeneration, decreasing the number of cotyledons used for transformation and reducing the time and labor required for transformation. This plasmid has been shown to be efficacious in both Hardy Pampas Grass ( E. Ravennae) and Tomato plants (S. Lycopersicum).

These products are sold under license by GoldBio and require a signed agreement before fulfillment. See license details below in the technical documentation.

pSuperAgro™ v4 was designed to include AcdS and GabT activity, driven by a single lac promotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.

AcdS activity is beneficial for higher callus induction frequency and suppresses callus browning phenomena. GabT activity decreases GABA content locally in plant cells and induces higher shoot regeneration, decreasing the number of cotyledons used for transformation and reducing the time and labor required for transformation. This plasmid has been shown to be efficacious in both Hardy Pampas Grass ( E. Ravennae) and Tomato plants (S. Lycopersicum).

These products are sold under license by GoldBio and require a signed agreement before fulfilment. See license details below in the technical documents.

pSuperAgro™ v1 was designed to include AcdS activity, driven by the lac promoter, which suppresses ethylene evolution during co-cultivation and significantly increases T-DNA transfer.

AcdS activity is beneficial for higher callus induction frequency and also suppresses callus browning phenomena. This plasmid has been shown to be efficacious in both Hardy Pampas Grass ( E. Ravennae) and Tomato plants (S. Lycopersicum).

pSuperAgro™ v1tet has been modified to provide tetracycline resistance instead of the original pSuperAgro™ V1’s gentamicin resistance. This allows the helper plasmid to be used in conjunction with our other GoldBio Agrobacterium strains, including the widely used GV3101 strain.

These products are sold under license by GoldBio and require a signed agreement before fulfillment. See license details below in the technical documents.

pSuperAgro™ v3 was designed to include GabT activity, driven by the lac promoter, which degrades gamma-aminobutyric acid (GABA) during co-cultivation. The reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.

GabT activity decreases GABA content locally in plant cells and induces higher shoot regeneration, decreasing the number of cotyledons used for transformation and reducing the time and labor required for transformation. This plasmid has been shown to be efficacious in both Hardy Pampas Grass ( E. Ravennae) and Tomato plants (S. Lycopersicum).

pSuperAgro™ v3tet has been modified to provide tetracycline resistance instead of the original pSuperAgro™ V3’s gentamicin with our other GoldBio Agrobacterium strains, including the widely used GV3101 strain.

These products are sold under license by GoldBio and require a signed agreement before fulfillment. See license details below in the technical documents.

pSuperAgro™ v4 was designed to include AcdS and GabT activity, driven by a single lac promoter, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.

AcdS activity is beneficial for higher callus induction frequency and also suppresses callus browning phenomena. GabT activity decreases GABA content locally in plant cells and induces higher shoot regeneration, decreasing the number of cotyledons used for transformation and reducing the time and labor required for transformation. This plasmid has been shown to be efficacious in both Hardy Pampas Grass ( E. Ravennae) and Tomato plants (S. Lycopersicum).

pSuperAgro™ v4tet has been modified to provide tetracycline resistance instead of the original pSuperAgro™ V4’s gentamicin resistance. This allows the helper plasmid to be used in conjunction with our other GoldBio Agrobacterium strains, including the widely used GV3101 strain.

These products are sold under license by GoldBio and require a signed agreement before fulfillment. See license details below in the technical documents.

GoldBio’s AGL-1 Agrobacterium Electrocompetent Cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in its recA recombination gene. This mutation stabilizes recombinant plasmids. AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection. A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted in the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Therefore, our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, maize, and other plants.

pSuperAgro™ v4 was designed to include AcdS and GabT activity, driven by a single lac promotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.

These products are sold under license by GoldBio and require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.

GoldBio’s C58C1 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. C58C1 has the chromosomal backbone of C58 but it has been cured of the Ti plasmid pTiC58. Our C58C1 strain harbors streptomycin and rifampicin resistance genes. C58C1 Competent cells are optimized for genetic transformation of plants such as Arabidopsis but can be used in many other plants.

pSuperAgro™ v4 was designed to include AcdS and GabT activity, driven by a single lac promotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.

These products are sold under license by GoldBio and require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.

GoldBio’s EHA105 Electrocompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene. EHA105 is ideal for genetic transformation of rice, tobacco and several other plants.

pSuperAgro™ v4 was designed to include AcdS and GabT activity, driven by a single lac promotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants. pSuperAgro™ v4tet has been modified to provide tetracycline resistance instead of the original pSuperAgro™ V4’s gentamicin resistance

These products are sold under license by GoldBio and require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.

GV2260 Agrobacterium chemically competent cells are a derivative of C58 Agrobacterium. GV2260 has the atu3300 gene in its linear chromosome and is a non-oncogenic Ti plasmid from which the T-region, including the entire TL- and TR-region, has been deleted and substituted by sequences derived from pBR322. pGV2260 is used as acceptor Ti plasmid and includes both carbenicillin and rifampicin resistance genes.

pSuperAgro™ v4 was designed to include AcdS and GabT activity, driven by a single lac promotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.

These products are sold under license by GoldBio and require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.

GoldBio’s GV3101 Agrobacterium Electrocompetent Cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our GV3101 strain harbors the C58 chromosomal backbone containing rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) harboring the gentamicin resistance. A functional T-DNA binary system can be built using our GV3101 strains as the T-DNA region has been deleted from the Ti plasmid and instead has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, tobacco, potato, soybeans and corn.

pSuperAgro™ v4 was designed to include AcdS and GabT activity, driven by a single lac promotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants. pSuperAgro™ v4tet has been modified to provide tetracycline resistance instead of the original pSuperAgro™ V4’s gentamicin resistance.

These products are sold under license by GoldBio and require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.

GoldBio’s LBA4404 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbor a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octoprine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes. Our LBA4404 strain can be used in genetic transformation of tomato, tobacco and other plants.

pSuperAgro™ v4 was designed to include AcdS and GabT activity, driven by a single lac promotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.

These products are sold under license by GoldBio and require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.

GoldBio’s AGL-1 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in its recA recombination gene. This mutation stabilizes recombinant plasmids. AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection. A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted from the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant S genome. Therefore, our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, maize, and other plants.

pSuperAgro™ v4 was designed to include AcdS and GabT activity, driven by a single lac promotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.

These products are sold under license by GoldBio and require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.

GoldBio’s C58C1 Chemically Competent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. C58C1 has the chromosomal backbone of C58 but it has been cured of the Ti plasmid pTiC58. Our C58C1 strain harbors streptomycin and rifampicin resistance genes. C58C1 Competent cells are optimized for genetic transformation of plants such as Arabidopsis but can be used in many other plants.

pSuperAgro™ v4 was designed to include AcdS and GabT activity, driven by a single lac promotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.

These products are sold under license by GoldBio and require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.

GoldBio’s EHA105 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene. EHA105 is ideal for genetic transformation of rice, tobacco and several other plants.

pSuperAgro™ v4 was designed to include AcdS and GabT activity, driven by a single lac promotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants. pSuperAgro™ v4tet has been modified to provide tetracycline resistance instead of the original pSuperAgro™ V4’s gentamicin resistance

These products are sold under license by GoldBio and require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.

GV2260 Agrobacterium chemically competent cells are a derivative of C58 Agrobacterium. GV2260 has the atu3300 gene in its linear chromosome and is a non-oncogenic Ti plasmid from which the T-region, including the entire TL- and TR-region, has been deleted and substituted by sequences derived from pBR322. pGV2260 is used as acceptor Ti plasmid and includes both carbenicillin and rifampicin resistance genes.

pSuperAgro™ v4 was designed to include AcdS and GabT activity, driven by a single lac promotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.

These products are sold under license by GoldBio and require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.

GoldBio’s GV3101 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our GV3101 strain harbors the C58 chromosomal backbone containing rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) harboring the gentamicin resistance. A functional T-DNA binary system can be built using our GV3101 strains as the T-DNA region has been deleted in the Ti plasmid and instead has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, tobacco, potato, soybeans and corn.

pSuperAgro™ v4 was designed to include AcdS and GabT activity, driven by a single lac promotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants. pSuperAgro™ v4tet has been modified to provide tetracycline resistance instead of the original pSuperAgro™ V4’s gentamicin resistance

These products are sold under license by GoldBio and require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.

GoldBio’s LBA4404 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbors a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octoprine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes. Our LBA4404 strain can be used in genetic transformation of tomato, tobacco and other plants.

pSuperAgro™ v4 was designed to include AcdS and GabT activity, driven by a single lac promotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.

These products are sold under license by GoldBio and require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.