Protocol - Ampicillin Stock Solution 100 mg/ml

Ampicillin Stock Solution - 100 mg/ml

Instructions

1. Weigh 1 g of Ampicillin (Ampicillin (Sodium), GoldBio Catalog # A-301 [CAS 69-52-3, mw.= 371.4]).
2. Add 10 ml of sterile H₂O. Dissolve completely.
3. Prewet a 0.22 μm syringe filter by drawing through 5-10 ml of sterile H₂O and discard water.
4. Sterilize Ampicillin Stock through the prepared 0.22 μm syringe filter.
5. Stock may be kept at -80°C for 1 year.
   • Note: Ampicillin solutions degrade 13% after one week at -20°C.