Tris (Tris Base)

Name: Tris (Tris Base)
SKU: T-400
Availability: InStock

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Product Description

GoldBio Tris has the highest purity available on the market. Short for Tris (hydroxymethyl) aminomethane (THAM), Tris is an organic compound often used in buffer solutions such as TAE or TBE for electrophoresis gels. Tris is highly soluble in water and is useful in the pH range 7.0-9.0. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer.

A Tris buffer solution can be made by mixing Tris with Tris-HCl. This prevents overshooting the pH and prevents the need to work with strong acids or bases.

Applications

Electrophresis (TAE/TBE/TE) buffers
SDS-PAGE
CTAB DNA extraction buffer
Enzymatic assays
Ellman's test

Storage/Handling

Store desiccated at room temperature.

Product Specifications

Catalog ID	T-400
Name(s)	Tris, Tris Buffer; 2-Amino-2-(hydroxymethyl)-1,3-propanediol
CAS #	77-86-1
Formula	C₄H₁₁NO₃
MW	121.14 g/mol
Grade	ULTRA PURE GRADE
Storage/Handling	Store desiccated at room temperature.
PubChem Chemical ID	6503

Technical Documentation

P Protocol

O Other

C Certificate of Analysis

S Safety Data Sheet

Antibody Binding Protocol - Protein A Agarose Beads

Protein A agarose resin column - Antibody binding protocol for a low pressure gravity column system.

Agarose Gel Preparation Protocol

Protocol for melting and pouring an agarose gel

Antibody Binding Protocol - Protein G Agarose Beads

Protein G agarose resin column - Antibody binding protocol for a low pressure gravity column system.

Ellmans Test Protocol

Quantitative Determination of Peptides by Sulfhydryl (-SH) Groups

Enzymatic Assay of L-Fucose Dehydrogenase

Enzymatic Assay of L-Fucose Dehyrogenase

TAE Stock Solution - 50X

50X TAE Stock Solution Buffer for gel electrophoresis.

TBE Stock Solution - 10X

10X TBE Stock Solution Buffer for gel electrophoresis.

TTE Stock Solution - 10X

10X Tris-TAPS-EDTA Stock Solution Buffer for gel electrophoresis

Tris Buffer Stock Solution

Tris Buffer Stock Solution - 1M

TE Buffer Stock Solution - 10X

10X TE Buffer for storage of nucleic acids such as DNA and RNA

One-Dimensional SDS-PAGE Protocol

Protocol for the preparation of an SDS-PAGE gel and electrophoresis of protein samples.

Low Melt Agarose Gel Preparation Protocol

Protocol for the preparation of agarose gels for electrophoresis using Low Melt Agarose.

Printable Buffer Chart - Arranged by Family

This ultimate buffer selection guide is a fantastic graphical display, showing common buffer pH ranges and their associated pKa. Buffers on this chart are also categorized by family. It's perfect to print out for your lab.

Printable Buffer pH Chart - Arranged In Order of pH

Refer to this printable buffer-pH chart for common buffers, associated pKa and pH range. Information on this chart is displayed in vibrant colors, and makes a great print-out for your lab.

User Guide for GoldBio Buffers

A user guide for the background, experimental use and technical discussion of GoldBio biological buffers.

Biological Buffer Selection Guide

The Biological Buffer Selection Guide includes catalog number, pH range, pKa, applications, stock solution protocol availability and comments for the most common biological buffers.

Guide: Commonly used Buffers

Guide: Commonly used Buffers contains name, pH range, applications, composition and other characteristics of buffers commonly used in the lab.

Sample COA - T-400

Sample Certificate of Analysis - Tris (Tris Base)
COA for example only. Actual tests may differ.

SDS (MSDS) - T-400

Safety Data Sheet - Tris (Tris Base)

Citations

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H-400-100 In stock	100 g	$ 51.00
H-400-250 In stock	250 g	$ 97.00
H-400-500 In stock	500 g	$ 151.00
H-400-1 In stock	1 kg	$ 240.00
H-400-2 In stock	2 x 1 kg	$ 408.00
H-400-5 In stock	5 kg	$ 882.00