Streptomycin Sulfate 100 mg/mL Solution
Streptomycin is an aminoglycoside antibiotic primarily effective against gram-negative bacteria and Mycobacterium species. It is commonly used in cell culture media to prevent bacterial contamination. It has also been used to precipitate nucleic acids during protein purification techniques and to study mechanisms of Streptomycin resistance.
Aminoglycoside antibiotics are composed of amino groups attached to glycosides. They bind the 30s ribosomal subunit, causing misreading of the mRNA sequence and inhibition of translocation. Consequently, protein synthesis is inhibited. Resistance to this antibiotic is conferred through mutations in the rpsL gene.
GoldBio’s antibiotic solutions are provided ready to use.
TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS AT GOLD BIOTECHNOLOGY LABS.
Streptomycin Sulfate in H2O
(100 mg/mL Premade Solution, 0.2 µm Sterile Filtered)
TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS
Formula: (C 21H39N7O12)2 · 3H2SO4
MW: 1457.38 g/mol
Storage/Handling: Store at -20°C.
PubChem Chemical ID: 19648
Antibiotic Testing Procedure
A brief explanation of the disk diffusion assay method used to test Gold Bio antibiotics
BRET (Bioluminescence Resonance Energy Transfer) Method
High Throughput Bioluminescence Resonance Energy Transfer (BRET) Method using Rluc and Coelenterazine by SW Gersting, et al. (2012)
BaF3 Proliferation Bioassay Protocol
BaF3 cell culture and Mitogenic assay for FGFR expressing BAF3 cells: by Dave Ornitz, Ornitz Lab. Washington University, St.Louis, MO
Antibiotic Treatments of Microbe-Contaminated Cell Cultures
Procedure on the use of different antibiotics to eliminate fungal, bacterial and mycoplasma contamination of cell lines
Sample COA - S-150
Sample Certificate of Analysis - Streptomycin Sulfate
COA for example only. Actual tests may differ.