Chromogenic Substrates Overview
Chromogenic substrates aid in the qualitative and quantitative detection of enzymes or other protein...
Salmon Gal (6-Chloro-3-indolyl-β-D-galactopyranoside) is used with IPTG as an alternative chromogenic substrate capable of detecting LacZ gene encoded ß-galactosidase activity for colony screening in colometric assays.
Unlike colony blue/white screening through X-Gal, nonrecombination results in a salmon color after salmon gal is cleaved by ß-galactosidase through hydrolysis. This contrast may be useful in comparative or secondary screenings.
Incorporating nitroblue tetrazolium salt (NBT) into the salmon gal reactions provides faster results with a clear, purple precipitate than in unaccompanied uses of salmon gal.
MOLECULAR BIOLOGY GRADE
PubChem Chemical ID: 10286594
Storage/Handling: Store dry at -15 °C, and protect from light.
|MOLECULAR BIOLOGY GRADE
|Store desiccated at -20°C. Protect from light.
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