pSuperAgro™ v1tet Plasmid (pBBRacdStet)

Introducing a new family of Agrobacterium helper plasmids, pSuperAgro™! These plasmids can greatly enhance gene transfer into various species of plants during Agrobacterium-mediated transformation. This increased efficiency provides a significant time and labor savings!

Sold as "Transformation Ready" DNA and offered at a low price to encourage rapid adoption of this exciting new technology. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research.

pSuperAgro™ v1 was designed to include AcdS activity, driven by the lac promoter, which suppresses ethylene evolution during co-cultivation and significantly increases T-DNA transfer.

AcdS activity is beneficial for higher callus induction frequency and also suppresses callus browning phenomena. This plasmid has been shown to be efficacious in both Hardy Pampas Grass (E. Ravennae) and Tomato plants (S. Lycopersicum).

pSuperAgro™ v1tet has been modified to provide tetracycline resistance instead of the original pSuperAgro™ V1’s gentamicin resistance. This allows the helper plasmid to be used in conjunction with our other GoldBio Agrobacterium strains, including the widely used GV3101 strain.

These products are sold under license by GoldBio and require a signed agreement before fulfillment. See license details below in the technical documentation.

Benefits

• Ethylene evolution suppression
• Higher callus induction frequency
• Reduced callus browning

Product Specifications

Concentration: 0.02 μg/μL in TE
Volume: 50 μL
Backbone size: 5809 bp
Vector type: Helper vector
Promoter: Lac
Bacterial Resistance(s): Tetracycline
Copy number: Low Copy

Vector Map

Sequence

GoldBio Vector Map and Sequence for pSuperAgro™ v1tet

Storage/Handling

pSuperAgro™ v1tet Plasmid DNA is at 0.02 μg/μL concentration in TE buffer.
Store all plasmids at -20°C.

Citations

• Nonaka, S., Sugawara, M., Minamisawa, K., Yuhashi, K. I., & Ezura, H. (2008). 1-Aminocyclopropane-1-carboxylate deaminase enhances Agrobacterium tumefaciens-mediated gene transfer into plant cells. Applied and Environmental Microbiology, 74(8), 2526-2528.
• Nonaka, S., Someya, T., Kadota, Y., Nakamura, K., & Ezura, H. (2019). Super-Agrobacterium ver. 4: improving the transformation frequencies and genetic engineering possibilities for crop plants. Frontiers in plant science, 10, 1204.