How Column Chromatography Works to Separate Proteins
Without a doubt, protein purification has been an important process in protein research, particularl...
Protein G HTC Agarose 4% Resin
Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains of native Protein G have been removed to ensure the maximum specific IgG binding capacity.
GoldBio offers rProtein G resins to isolate and purify classes, subclasses and fragments of immunoglobulins from cell culture media and biological fluids. Rapid purification and high yield of purified immunoglobulin are obtainable by this method.
Protein G is covalently bound to highly cross-linked agarose beads, providing a very stable bond that can greatly minimize leakage of the Protein G and allowing for reuse of the affinity resin in several purification steps. This resin works well in High Flow conditions.
HTC beads are high throughput beads designed to meet the demand for industrial process separation. Their rigidity and mechanical resistance permits high flow rates with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly cross-linked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity chromatography and base media support for producing IEX (Ion Exchange) and hydrophobic interaction chromatography resins.
Protein G HTC agarose beads are suitable for use with FPLC or HPLC, and can withstand pressures up to 300 kPa.
Storage/Handling: Store at 4°C. Do NOT freeze.
Catalog ID | P-430 |
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Storage/Handling | store at 4°C. Do NOT freeze. |
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