Troubleshooting Culture Media Clumping and Vitrification
Researchers usually encounter two problems when working with gelling agents. They are clumping, and...
GoldBio Plant agar is the preferred choice in Plant cell and Tissue culture as a general purpose agar and combines good quality with a favorable price. Agar is derived from red algae and is comprised of a mix of large polysaccharide agarose molecules and smaller, heterogeneous molecules called agaropectin.
Plant agar can be used in a minimal concentration of 5.5 g/L to to obtain a solid gel.
agar-agar, Gum agar;
Gel Strength: >1100 g/cm 2
Storage/Handling: Store at room temperature.
Preparation: Add Plant Agar to media mix at desired concentration (minimum 5.5 g/L). Autoclave media at 121°C for 20 minutes. Remove from the autoclave immediately and cool to 50°C before pouring plates.
|Storage/Handling||Store at room temperature.|
Potato-Agrobacterium Transformation using Kanamycin, Timentin and Zeatin - by Hui Duan et al. (2012)
Protocol for yeast plate preparation and screening of transformed yeast.