PIPES, Free Acid
PIPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.1 – 7.5. PIPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. PIPES has wide variety of applications and is commonly used in cell culture media, in protein crystallization, as a running buffer in gel electrophoresis, and as an eluent in isoelectric focusing and chromatography. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. It is suitable for use in the bicinchoninic acid (BCA) assay. Solubility of PIPES increases when the free acid is converted to the sodium salt.
Buffers are made by adding base solution to PIPES free acid and titrating to the desired pH.
PIPES, Free Acid
HIGH PURITY GRADE
MW: 302.37 g/mol
Storage/Handling: Store in a dry and well ventilated place.
PubChem Chemical ID: 79723
|Grade||HIGH PURITY GRADE|
|Storage/Handling||Store desiccated at room temperature.|
This ultimate buffer selection guide is a fantastic graphical display, showing common buffer pH ranges and their associated pKa. Buffers on this chart are also categorized by family. It's perfect to print out for your lab.
Refer to this printable buffer-pH chart for common buffers, associated pKa and pH range. Information on this chart is displayed in vibrant colors, and makes a great print-out for your lab.
A user guide for the background, experimental use and technical discussion of GoldBio biological buffers.
The Biological Buffer Selection Guide includes catalog number, pH range, pKa, applications, stock solution protocol availability and comments for the most common biological buffers.
Sample Certificate of Analysis - PIPES, Free Acid
COA for example only. Actual tests may differ.