24 Real Lab Hacks from Researchers Like You
Life hacks are all over the Internet, but laboratory hacks are more difficult to find. After being...
PBS (phosphate buffered saline) is a common buffer solution used in the laboratory. It is an isotonic and non-toxic buffer that is meant to mimic the osmolarity and ion concentrations of the human body. PBS is used for immunoassays, for sample dilution, for protein purification, and as a wash buffer in cell cultures. PBS tablets are premeasured tablets for convenient preparation of 1X PBS solution at a pH of 7.3 – 7.5. PBS buffer contains 10mM phosphate buffer, 137mM sodium chloride, and 2.7mM potassium chloride.
Each tablet prepares 100 mL of a 1X PBS solution.
Phosphate Buffered Saline Tablets
Storage/Handling: Store in a dry and well-ventilated place.
|Store desiccated at room temperature.
High Throughput Bioluminescence Resonance Energy Transfer (BRET) Method using Rluc and Coelenterazine by SW Gersting, et al. (2012)
Protein A agarose resin column - Antibody binding protocol for a low pressure gravity column system.
IPTG Induction & Extraction of Proteins - by Arur and Nayak. Schedl Lab, Wash U. St. Louis
Procedure for noncovalent protein internalization using β-Gal and X-Gal, by Wang et al. (2006).
Preparation of Proteins for Western Blotting of Monolayer Cells
A protocol for the use of a methylumbelliferyl substrate for identifying yeast isolate enzymes by Bobey and Ederer (1981).
Use of RIPA Buffer to prepare cell lysates for protein assays, protein purification and other downstream applications.
This ultimate buffer selection guide is a fantastic graphical display, showing common buffer pH ranges and their associated pKa. Buffers on this chart are also categorized by family. It's perfect to print out for your lab.
Refer to this printable buffer-pH chart for common buffers, associated pKa and pH range. Information on this chart is displayed in vibrant colors, and makes a great print-out for your lab.
A user guide for the background, experimental use and technical discussion of GoldBio biological buffers.
Guide: Commonly used Buffers contains name, pH range, applications, composition and other characteristics of buffers commonly used in the lab.
Sample Certificate of Analysis - PBS (Phosphate Buffered Saline) Tablets
COA for example only. Actual tests may differ.
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