pACYC Plasmid

The pACYC plasmid series is another set of commonly used cloning vectors in molecular biology and biotechnology. pACYC184 has a p15A origin of replication with chloramphenicol and tetracycline resistance genes. Unlike the pUC or pBR325 vectors, pACYC184 plasmids are characterized by a low copy number. This means fewer copies of the plasmid are present in each bacterial cell, which can be advantageous for cloning and expressing genes that are toxic to the host cells when present in high quantities. pACYC184 has a small size of 4245 bp and is suitable for cloning small to medium-sized DNA fragments. pACYC plasmids are especially useful in experiments where multiple plasmids are required within the same cell, or when cloning genes that are deleterious to host cells at high copy numbers.

Benefits

• Low Copy cloning
• Origin of Replication: p15A origin. This allows it to be maintained in the same cell as plasmids with the ColE1 origin, enabling co-transformation and expression of multiple plasmids in a single cell.
• Resistance Genes: Chloramphenicol and Tetracycline
• Small size: The plasmid is 4245 bp, suitable for cloning small to medium-sized DNA fragments.
• Versatility in experiments where multiple plasmids are required within the same cell, or when cloning genes that are deleterious to host cells at high copy numbers.

Product Specifications

Concentration: 0.02 μg/μL in TE
Volume: 50 μL
Backbone size: 4245 bp
Vector type: Cloning vector
Bacterial Resistance(s): Tetracycline, Chloramphenicol
Copy number: Low Copy
Genbank Assession Number: X06403.1

Vector Map

Sequence

GoldBio Vector Map and Sequence for pACYC

Storage/Handling

pACYC DNA is at 0.02 μg/μL concentration in TE buffer.
Store all plasmids at -20°C.