How to Choose the Right Biological Buffer for My Experiment
Choosing the right biological buffer for your experiment can be tricky. The buffer choosing proces...
MOPSO is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer, structurally similar to MOPS, that is useful for a pH range of 6.2 – 7.6. It is commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification with chromatography. Due to low ionic mobility, MOPSO is regarded as an excellent buffer for use in capillary electrochromatography. MOPSO lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. It does form a complex with some organotin(IV) molecules that are being explored as antitumor agents. MOPSO also interacts with the peptide backbone of bovine serum albumin (BSA) to stabilize BSA against thermal denaturation.
MOPSO free acid can be mixed with MOPSO sodium salt to acquire desired pH. Alternatively, MOPSO free acid can be titrated with sodium hydroxide to attain desired pH.
ULTRA PURE GRADE
MW: 225.26 g/mol
PubChem Chemical ID: 109333
Storage/Handling: Store at room temperature.
|ULTRA PURITY GRADE
|Store desiccated at -20°C.
This ultimate buffer selection guide is a fantastic graphical display, showing common buffer pH ranges and their associated pKa. Buffers on this chart are also categorized by family. It's perfect to print out for your lab.
Refer to this printable buffer-pH chart for common buffers, associated pKa and pH range. Information on this chart is displayed in vibrant colors, and makes a great print-out for your lab.
A user guide for the background, experimental use and technical discussion of GoldBio biological buffers.
The Biological Buffer Selection Guide includes catalog number, pH range, pKa, applications, stock solution protocol availability and comments for the most common biological buffers.
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