All About the Turbidity-Based Lysozyme Activity Assay
Lysozyme is an enzyme that breaks down peptidoglycan, and it is often used in protein purification,...
Lysozyme is an enzyme capable of breaking down the bacterial cell wall, and is typically used to enhance protein extractions or nucleic acid extractions. It works by hydrolyzing the 1,4-β-linkages located between the N-acetylmuramic acid and the N-acetyl-D-glucosamine residues in peptidoglycan.
Gram-positive cells have an increased susceptibility to the actions of lysozyme since gram-positive cells have a higher quantity of peptidoglycan in the cell wall. Gram-negative cells are less vulnerable, but can still be hydrolyzed in the presence of EDTA.
Lysozyme, Egg White
MOLECULAR BIOLOGY GRADE
Storage/Handling: Store at -20°C. Avoid freeze/thaw cycles
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Protocol for the preparation of an SDS-PAGE gel and electrophoresis of protein samples.
Sample Certificate of Analysis - Lysozyme, Egg White
COA for example only. Actual tests may differ.