Glyoxal (Low Density) 6% Agarose Beads, Crosslinked

Product Description

Biomolecules can be immobilized by binding them to a support under conditions that will then determine the characteristics of the complex. Immobilization is a technique that binds a ligand (enzyme, antibody, affinity proteins like Protein A or Protein G) to a support, giving high stability and making it easier to reuse the immobilized molecule.

Glyoxal beads allow a covalent binding of agarose to lysine amino groups (similar orientation to CNBr). Glyoxal agarose beads are ready to use and require no activation. The resin has a high amount of aldehydes groups (15-25 µmoles Glyoxal / ml gel) providing a very good option to conjugate affinity ligands improving the stability of the ligand.

The choice of Glyoxal or Aminoethyl will depend on the biomolecule to be immobilized, the accessibility of the reactive groups, and the direction/ orientation required for the binding to the support.

Glyoxal 6% agarose beads are suitable for use with FPLC, but with pressures no more than 20 kPa. Too much pressure or too fast of a flow rate will result in diminished performance.

Choosing High and Low Density Agarose Beads

High Density Resin

Low Density Resin

High Crosslinking

Low Crosslinking

Best for smaller proteins

Best for larger proteins (e.g. antibodies)

Best for repeated use or FPLC systems

Best for batch incubations or gravity flow

Small pores

Large pores

Stronger covalent binding of ligands improves ligand stability

Weaker covalent binding improves ligand mobility after immobilization

Storage/Handling

Store at 4°C. Do not freeze.

Product Specifications

Catalog ID G-302
Name(s) Glyoxal (Low Density) 6% Agarose Beads, Crosslinked
Bead Size Spherical, Standard: ~50-150 μm (approx.)
Cross-linking Yes
Binding/Loading Capacity 5-10 (mg BSA/ml gel)
Activity 15-25 (μmol glyoxyl/ml gel)
Agarose % 6% agarose
Chemical Stability Stable in all commonly used reagents.
Temperature Stability Autoclavable at 120°C for 20 min in H2O
Storage Buffer 20% Ethanol
Storage/Handling store at 4°C. Do NOT freeze.

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