Conjugating Ligands to Agarose Beads for Affinity Purification
Affinity purification is a frequently used technique for purifying proteins. During affinity purific...
Glyoxal HTC 6% HIGH Density Agarose Beads
Product Description
Glyoxal HTC 6% HIGH Density Agarose beads allow covalent binding of agarose to lysine amino groups (similar orientation to CNBr).
This product is adequate to work in large scale process (High Pressure). The resin has a high amount of aldehyde groups (40-60 µmoles Glyoxal / ml gel) providing multiple binding points and high immobilized ligand stability. The resin has been manufactured using 6% HTC agarose beads, and the pore size is adequate to work with enzymes, proteins & small ligands. The stability at high flow is adequate for large scale process.
Glyoxal 6% HTC agarose beads are suitable for use with FPLC or HPLC, and can withstand pressures up to 300 kPa.
Storage/Handling: Store at 4°C. Do not freeze.
Product Specifications
| Catalog ID | G-312 |
|---|---|
| Name(s) | Glyoxal HTC 6% HIGH Density Agarose Beads |
| Bead Size | Spherical, Standard: ~50-150 μm (approx.) |
| Cross-linking | Highly crosslinked |
| Activity | 40-60 (μmol glyoxyl/ml gel) |
| Agarose % | 6% agarose |
| Chemical Stability | Stable in all commonly used reagents. |
| Temperature Stability | Autoclavable at 120°C for 20 min in H2O |
| Exclusion Limit | >4x106 |
| Storage Buffer | 20% Ethanol |
| Storage/Handling | Store at 4°C. Do NOT freeze. |
Technical Documentation
P
Protocol
O
Other
C
Certificate of Analysis
S
Safety Data Sheet
