Erythromycin is a macrolide antibiotic isolated from Streptomyces erythreus. It displays strong efficacy against gram-positive cocci and bacilli and some mycobacteria, mycoplasma, and spirochetes. In addition to its antimicrobial properties, erythromycin is a motilin receptor agonist that has been shown to inhibit respiratory glycoconjugate secretion in vitro. It has also been used to select for recombinant Lactococcus lactis MG1363 strains in microbiological applications.
Macrolide antibiotics are composed of a macrocyclic lactone ring attached to one or more deoxy sugars. They inhibit bacterial protein synthesis by irreversibly binding the P site of the 50s ribosome, preventing peptide bond formation and translocation.
Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.
MW: 733.93 g/mol
Storage/Handling: Store at 4°C. Soluble in EtOH. Poor solubility in water.
PubChem Chemical ID: 12560
|Storage/Handling||Store at 4°C.|
Broth Microdilution and Disk Diffusion
Broth Microdilution & Disk Diffusion tests of Erythromycin and Clindamycin by Jorgensen et. al. (2011)
Antibiotic Treatments of Microbe-Contaminated Cell Cultures
Procedure on the use of different antibiotics to eliminate fungal, bacterial and mycoplasma contamination of cell lines