E-64 (trans-Epoxysuccinyl-L-leucylamido(4-guanidino)butane) irreversibly inhibits cysteine proteases without affecting other enzymes that contain cysteine residues. E-64 is capable of inhibiting bromelain, trypsin, papain, staphopain, calpain, collagenase, ficain, cathepsin B, H and L. Inhibition of serine proteases is not seen with E-64, excepting trypsin.
E-64 is usually used in a concentration range of 1 to 10μM. It is widely used for in vivo studies due to its cell permeability, low toxicity, and potency. E-64 is being investigated for the treatment of diseases caused by cysteine protease activity. It has been observed to inhibit programmed cell death and restore antigen and mitogen activity related to normal proliferative responses in T cells obtained from HIV+ donors.
MOLECULAR BIOLOGY GRADE
MW: 357.41 g/mol
Storage/Handling: Store at -20°C.
PubChem Chemical ID: 123985
|MOLECULAR BIOLOGY GRADE
|Store at -20°C.