Description
DNase I, Bovine Pancreas (Lysate Tested), >2000 Kunitz U/mg is a high specific activity endonuclease for applications requiring efficient DNA degradation with minimal enzyme mass. Its high enzymatic activity allows researchers to achieve effective digestion while adding less protein to reaction mixtures, making it well suited for sensitive molecular biology procedures where minimizing contaminants is important. Lysate testing provides additional confidence for use in DNA removal applications involving recombinant proteins and other biological samples.
DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.
Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease, which splits phosphodiester linkages preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3' position and a phosphate group at the 5' position. The optimum pH is 7.8.
DNase I is activated by bivalent metals and inhibited by chelating agents such as EDTA and SDS. DNase I can be stabilized against proteolytic digestion by 5mM Ca2+.
Product Specifications
DNase I
Deoxyribonuclease I
DNase I, Bovine Pancreas >2000 Kunitz U/mg
Unit Definition: One Kunitz Unit will produce an A260 of 0.001 per minute at pH 5.0 at 25°C using DNA, type I or III as the substrate.
MOLECULAR BIOLOGY GRADE
Storage/Handling: Store desiccated at -20°C.
Allow product to come to room temperature before opening.
For greatest stability, it is important that DNase to be dissolved at a concentration of at least 1 mg/ml in 50% glycerol with 20mM Tris-HCl, pH 7.5, and 1mM MgCl2.
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