Description
GoldBio's D-Biotin is a high-purity, water-soluble vitamin commonly used as a cofactor in cell culture, microbiological media, and enzyme-based research applications. It also serves as a key reagent in biotin-streptavidin affinity systems, where its exceptionally strong and specific binding to streptavidin or avidin enables protein purification, molecular labeling, and assay development.
D-Biotin is an essential coenzyme for several carboxylase enzymes involved in fatty acid synthesis, amino acid metabolism, and gluconeogenesis. In cell culture and microbiological applications, it supports normal cellular metabolism and growth by participating in these biochemical pathways. It is routinely added to defined media and vitamin supplements for bacterial, yeast, and mammalian cell culture.
In molecular biology and biochemistry, D-Biotin can be incorporated into proteins, peptides, and other biomolecules through compatible chemical or enzymatic biotinylation methods. The resulting biotinylated molecules can then be selectively captured or detected using streptavidin- or avidin-based systems. These biotinylated molecules can then be selectively captured or detected using streptavidin- or avidin-based systems. D-Biotin plays an important role in affinity purification, immunoassays, ELISA, Western blotting, labeling, and diagnostic assay development.
Common Applications
(Click each for more information)
Cell Culture Media Supplementation
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Purpose: To support normal cellular metabolism and growth in defined cell culture media.
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How It Works: D-Biotin serves as an essential cofactor for biotin-dependent carboxylase enzymes involved in fatty acid synthesis, amino acid metabolism, and gluconeogenesis. Supplementing culture media with biotin helps maintain normal cellular function and proliferation.
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Applications: Mammalian cell culture, stem cell research, primary cell culture, and serum-free or chemically defined media.
Eagle, H. (1955). The minimum vitamin requirements of the L and HeLa cells in tissue culture. Journal of Experimental Medicine, 102(5), 595–600.
Microbiological Media Preparation
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Purpose: To provide an essential vitamin required for the growth of biotin-dependent microorganisms.
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How It Works: Many bacteria and yeast require biotin as a metabolic cofactor for carboxylase enzymes. Supplementing microbiological media ensures adequate vitamin availability for growth and physiological studies.
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Applications: Bacterial culture, yeast culture, microbial physiology, nutritional studies, and fermentation research.
Said, H. M. (2002). Biotin: The forgotten vitamin. American Journal of Clinical Nutrition, 75(2), 179–180.
Affinity Purification
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Purpose: To support the preparation and use of biotinylated biomolecules in affinity capture and purification systems.
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How It Works: D-Biotin can be incorporated into a target molecule using compatible chemical or enzymatic methods. The biotinylated target can then be captured on an avidin- or streptavidin-containing support through the high-affinity biotin-binding interaction.
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Applications: Affinity chromatography, protein purification, nucleic acid purification, and biomolecule immobilization.
Green, N. M. (1975). Avidin. Advances in Protein Chemistry, 29, 85–133.
Biomolecule Labeling and Detection
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Purpose: To facilitate sensitive detection of proteins, antibodies, nucleic acids, and other biomolecules.
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How It Works: Biotinylated biomolecules are detected using streptavidin- or avidin-conjugated enzymes, fluorophores, or other reporter molecules, providing highly specific signal amplification.
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Applications: ELISA, Western blotting, immunohistochemistry, fluorescence microscopy, and molecular diagnostics.
Wilchek, M., & Bayer, E. A. (1990). Introduction to avidin-biotin technology. Methods in Enzymology, 184, 5–13.
Protein Interaction and Pull-Down Assays
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Purpose: To isolate and study protein-protein, protein-DNA, and protein-RNA interactions.
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How It Works: Biotinylated molecules are immobilized on streptavidin supports, allowing interacting partners to be captured, enriched, and analyzed while minimizing non-specific binding.
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Applications: Pull-down assays, chromatin studies, interactome analysis, RNA-binding studies, and proteomics.
de Boer, E., Rodriguez, P., Bonte, E., et al. (2003). Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice. Proceedings of the National Academy of Sciences, 100(13), 7480–7485.
Key Benefits
· Dual Research Utility: Functions as both an essential biological cofactor and a key reagent for biotin-streptavidin affinity technologies, making it suitable for a wide range of life science applications.
· High-Affinity Streptavidin Binding: Forms one of the strongest known non-covalent biological interactions with streptavidin and avidin, enabling highly specific capture, purification, and detection of biotinylated biomolecules.
· Supports Healthy Cell Growth: Provides an essential vitamin required for normal cellular metabolism and proliferation in mammalian, bacterial, and yeast culture systems.
· Versatile Research Reagent: Compatible with applications including cell culture, affinity purification, molecular labeling, immunoassays, protein interaction studies, and diagnostic assay development.
· Reliable Performance Across Multiple Disciplines: Widely used in molecular biology, biochemistry, microbiology, cell biology, and proteomics, making it a trusted reagent for diverse research applications.
Storage/Handling
Store at 4°C.
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