Cytokines and Colony Stimulating Factors
In the ever-evolving world of growth factors, the Cytokine class can potentially encompass nearly ev...
GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!
Recombinant Murine Colony Stimulating Factor 3 (granulocyte)
Granulocyte Colony-Stimulating Factor, G-CSF
Colony Stimulating Factor 3 (CSF3), formerly called granulocyte colony stimulating factor (GCSF), is a pleiotropic cytokine. It is mainly produced by monocytes and macrophages upon activation by endotoxin, TNFα and IFNγ. Many cell types secreted this protein after LPS, IL1 or TNFα activation, including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells. Various carcinoma cell lines and myeloblastic leukemia cells can express CSF3 constitutively. CSF3 is cytokine that acts in hematopoiesis by controlling the production, differentiation and function of 2 related white cell populations of the blood, the granulocytes and the monocytesmacrophages. In addition, it may function in some adhesion or recognition events at the cell surface. The murine CSF3 cDNA encodes a 208 amino acid residue precursor protein containing a 30 amino acid residue signal peptide that is proteolytically cleaved to generate the 178 amino acid residue mature protein. Murine CSF3 is 73% identical at the amino acid level to human CSF3 and the two proteins show species crossreactivity.
Sterile Filtered White lyophilized powder. Purified and tested for use in cell culture.
MW:~18.9 kDa (178 amino acids)
Purity:>98% by SDS-PAGE and HPLC analyses.
Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NFS60 cells is less than 0.05 ng/ml, corresponding to a specific activity of >2.0×107 IU/mg.
Storage/Handling:Store desiccated at -20°C.
NOT FOR HUMAN USE!
Additional resources for this protein may be found at:
|Store desiccated at -20°C.
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