Cytokines and Colony Stimulating Factors
In the ever-evolving world of growth factors, the Cytokine class can potentially encompass nearly ev...
GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!
Recombinant Human Colony Stimulating Factor 2 (granulocyte-macrophage)
MGI-1GM (Macrophage granulocyte inducer-1GM)
Colony Stimulating Factor 2 (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types, including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts, in response to cytokine or immune and inflammatory stimuli. CSF2 is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, CSF2 is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. It can induce human endothelial cells to migrate and proliferate and can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. CSF2 is species specific and human CSF2 has no biological effects on mouse cells.
Formulation: Sterile filtered white lyophilized powder. Purified and tested for use in cell culture.
Sequence: APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE
MW: ~14.6 kDa (128 amino acid)
Purity: >96% by SDS-PAGE and HPLC analyses
Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a assay using human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of >1.0 × 107 IU/mg.
Accession Number: P04141
HGNC ID: 2434
Source: Escherichia coli
Storage/Handling: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage. The reconstituted sample can be apportioned into working aliquots and stored at -80 °C for up to 6 months. Avoid repeated freeze/thaw cycles.
Reconstitution: The sample should be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a siliconized tube using PBS that contains a 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C. Stock solutions should be aliquoted and stored at -80°C. Further dilutions should be made in appropriate buffered solutions containing BSA or serum.
Additional resources for this product may be found at:
NOT FOR HUMAN USE!
|Storage/Handling||Store desiccated at -20°C.|