Chromogenic Substrates Overview
Chromogenic substrates aid in the qualitative and quantitative detection of enzymes or other protein...
Bluo-Gal is used as an alternative to X-Gal, and produces a darker blue color. Bluo-Gal is hydrolyzed by β-galactosidase to produce a blue precipitate. This precipitate is insoluble in alcohol and xylenes, thus making it suitable for immunoblotting and immunocytochemical assays. Bacterial colonies containing active β-galactosidase produce blue colonies when grown on plates containing Bluo-Gal.
Bluo-Gal is commonly used in conjunction with IPTG (GoldBIo #I2481) for blue-white screening.
MOLECULAR BIOLOGY GRADE
MW: 374.18 g/mol
Purity: >99% by HPLC
Soluble in dimethylformamide (DMF)
Storage/Handling: Store at -15°C, and protect from light.
PubChem Chemical ID: 44161406
|MOLECULAR BIOLOGY GRADE
|Store at -20°C. Protect from light.
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