BLUEstain™ Low Range Protein Ladder, 2-40 kDa
Product Description
The BLUEstain™ Low Range Protein Ladder, 2 to 40 kDa is a ready-to-use three-color protein standard with 6 pre-stained proteins covering a range of molecular weights from 2 to 40 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 10 kDa and 40 kDa respectively) when separated on SDS-PAGE.
BLUEstain™ Low Range Protein Ladder is designed for monitoring protein separation during SDS-PAGE electrophoresis, verification of Western transfer efficiency on membranes (PVDF or nitrocellulose) and for approximating the molecular weight of small proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute or add reducing agent before loading.
Contents:
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20mM
Tris-phosphate, pH 7.5 at 25°C, 2% SDS, 10mM Dithiothreitol, 3.6 M Urea,
and 15% (v/v) Glycerol).
Features
- Ready-to-use! No dilutions necessary.
- Two reference bands — 40 kDa (red) and 10 kDa (green).
- Enhanced band clarity for proteins below 10 kDa.
- Versatile application across various gel systems, including Tris-Tricine, Tris-Glycine, and Bis-Tris gels.
- 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
- 1.5~2.5 μl per well for general Western transferring.
- Apply more for thicker (>1.5 mm) or larger gel.
Storage/Handling
Store at 4°C for 3 months. Store at -20°C for 24 months.
This product is stable for shipping at ambient temperatures.
Product Specifications
| Name(s) | BLUEstain™ Low Range Protein ladder, 2-40 kDa |
|---|---|
| Catalog ID | P002 |
| Storage/Handling | Store at 4°C for 3 months. Store at -20°C for 24 months. |
