Bis-Tris, also known as bis-Tris methane, is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a bis(2-hydroxyethyl)amine and Tris family buffer that is useful for a pH range of 5.8 – 7.2. Bis Tris is a common component of many buffer systems for various types of electrophoresis. Bis-Tris may form a complex with some common metals, such as Cu(II) and Pb(II), and formation constants should be taken into account when using this buffer in a solution containing metal ions. Bis-Tris can be used as a substitute for the highly toxic buffer cacodylate.
Bis-Tris, Ultra Pure
Bis-(2-Hydroxyethyl) Amino-Tris(Hydroxymethyl) Methane
MW: 209.24 g/mol
Storage/Handling: Keep in a dry and in a well-ventilated place.
PubChem Chemical ID: 81462
|Storage/Handling||Store at room temperature.|
Printable Buffer Chart - Arranged by Family
This ultimate buffer selection guide is a fantastic graphical display, showing common buffer pH ranges and their associated pKa. Buffers on this chart are also categorized by family. It's perfect to print out for your lab.
Printable Buffer pH Chart - Arranged In Order of pH
Refer to this printable buffer-pH chart for common buffers, associated pKa and pH range. Information on this chart is displayed in vibrant colors, and makes a great print-out for your lab.
User Guide for GoldBio Buffers
A user guide for the background, experimental use and technical discussion of GoldBio biological buffers.
Biological Buffer Selection Guide
The Biological Buffer Selection Guide includes catalog number, pH range, pKa, applications, stock solution protocol availability and comments for the most common biological buffers.
Guide: Commonly used Buffers
Guide: Commonly used Buffers contains name, pH range, applications, composition and other characteristics of buffers commonly used in the lab.
Sample COA - B-020
Sample Certificate of Analysis - Bis-Tris
COA for example only. Actual tests may differ.