Description
- 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
- 1.5~2.5 μl per well for general Western transferring.
- Apply more for thicker (>1.5 mm) or larger gel.
- Ready-to-use! No dilutions necessary
GoldBio’s BLUEstain™ 2 Protein Ladder (5–245 kDa) is a ready-to-use, pre-stained protein marker designed for accurate molecular weight estimation and efficient gel electrophoresis monitoring.
Featuring 13 distinct pre-stained bands spanning a broad size range, this tri-color standard enables you to track protein migration in real-time, validate transfer efficiency during western blotting, and normalize protein loading across lanes with clarity.
Ideal for SDS-PAGE, western blotting, and reproducible gel imaging, BLUEstain™ 2 enhances lab efficiency while supporting consistent, publication-quality results. Trusted for its accuracy and ease of use, it’s an essential reference tool in modern proteomics and molecular biology.
Contents:
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20mM Tris-phosphate, pH 7.5 at 25°C, 2% SDS, 10mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol).
Storage/Handling:
Store at 4°C for 3 months. Store at -20°C for 24 months.
This product is stable for shipping at ambient temperatures.
Common Applications
(Click each for more information)
Molecular Weight Estimation in SDS-PAGE and Western Blotting
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Purpose: To estimate the size of unknown proteins by comparing them to a reference ladder during electrophoresis.
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How It Works: BLUEstain™ 2 Protein Ladder contains 13 pre-stained polypeptides that migrate according to molecular weight, allowing alignment of unknown protein bands with reference standards during or after electrophoresis.
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Applications: Protein expression analysis, purification workflows, and antibody validation.
Ladner, C. L., Yang, J., Turner, R. J., & Edwards, R. A. (2004). Visible fluorescent detection of proteins in polyacrylamide gels without staining. Analytical Biochemistry, 326(1), 13–20.
Monitoring Gel Electrophoresis Progress in Real-Time
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Purpose: To visually track protein migration during SDS-PAGE runs.
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How It Works: The pre-stained bands remain visible throughout electrophoresis, allowing researchers to monitor separation progress without post-run staining.
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Applications: Optimizing electrophoresis run times and resolving low- or high-molecular-weight proteins.
Kurien, B. T., & Scofield, R. H. (2006). Western blotting. Methods, 38(4), 283–293.
Transfer Efficiency Verification in Western Blotting
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Purpose: To confirm successful transfer of proteins from SDS-PAGE gels onto blotting membranes.
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How It Works: Visible pre-stained bands provide immediate confirmation that proteins across the molecular weight range have transferred effectively.
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Applications: Western blot quality control and troubleshooting uneven or incomplete transfers.
Aldridge, G. M., Podrebarac, D. M., Greenough, W. T., & Weiler, I. J. (2008). The use of total protein stains as loading controls: an alternative to high-abundance single-protein controls in semi-quantitative immunoblotting. Journal of Neuroscience Methods, 172(2), 250–254.
Protein Loading Normalization Across Lanes
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Purpose: To visually confirm consistent loading volumes and protein input across gel lanes.
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How It Works: Ladder band intensity and alignment serve as reference indicators when comparing relative protein expression or sample loading consistency.
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Applications: Semi-quantitative Western blotting, reproducibility checks, and comparative protein analysis.
Ghosh, R., Gilda, J. E., & Gomes, A. V. (2014). The necessity of and strategies for improving confidence in the accuracy of western blots. Expert Review of Proteomics, 11(5), 549–560.
Imaging and Documentation Standardization
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Purpose: To provide consistent visual markers for imaging calibration, lane alignment, and exposure normalization.
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How It Works: Multicolor pre-stained bands remain visible during imaging, enabling consistent positioning and exposure settings across gels and instruments.
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Applications: Figure preparation, reproducibility studies, and automated gel documentation workflows.
Gilda, J. E., & Gomes, A. V. (2013). Stain-free total protein staining is a superior loading control to β-actin for Western blots. Analytical Biochemistry, 440(2), 186–188.
Benefits:
- Real-Time Electrophoresis Monitoring: Eliminates guesswork during gel runs with visible migration tracking.
- Transfer Verification: Easily confirms blotting success across molecular weights.
- Reproducible Results: Improves consistency in protein sizing and normalization across experiments.
- Streamlined Workflow: Supplied in ready-to-use format to reduce prep time.
- Educational Utility: Excellent for demonstrating protein analysis principles in teaching labs.
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