5-Fluorouracil (5-FU) is a uracil analog that has a fluorine atom on the fifth carbon position. It acts as a strong anti-cancer agent where its metabolization disrupts both RNA and DNA synthesis. 5-FU metabolites, Fluorodeoxyuridine triphosphate (FdUTP) and fluorouridine triphosphate (FUTP), imitate uracil and are incorporated into RNA strands which effectively inhibits further synthesis.
Inhibition of DNA synthesis by 5-FU occurs through thymidylate synthase inhibition. A metabolite produced by 5-FU metabolism, fluorodeoxyuridine triphosphate (FdUTP), bind to the nucleotide binding site of thymidylate synthase. Halting this enzyme’s activity significantly diminishes deoxythymidine monophosphate (dTMP) production which consequently depletes dTMP within an organism.
MW: 130.08 g/mol
Storage/Handling: Store desiccated at room temperature.
PubChem Chemical ID: 3385
|Store desiccated at room temperature.