4-Methylumbelliferyl-α- D-mannopyranoside is a fluorogenic substrate for α-D-mannosidase, yielding a blue fluorescent solution upon cleavage. It is used for determination of lectin in a very dilute solution by fluorescence quenching, in studies of the kinetics of saccharide binding to the dimer form of concanavalin A or for diagnosis of alpha-mannosidosis by measuring α-mannosidase in plasma.
MOLECULAR BIOLOGY GRADE
MW: 338.32 g/mol
Storage/Handling: Store at -20°C. Protect from light.
1. T. Yoshihisa, et al., Solubilization and purification of alpha-mannosidase, a marker enzyme of vacuolar membranes in Saccharomyces cerevisiae. J. Biol. Chem. 1988, 263, 5158.
2. P.K. Datta, S.C. Bera, Determination of lectin in very dilute solution with umbelliferyl sugars by fluorescence quenching. Anal. Chim. Acta 1989, 220, 225.
|Grade||MOLECULAR BIOLOGY GRADE|
|Storage/Handling||Store at -20°C. Protect from light.|