MES, Sodium Salt

MES sodium salt is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 5.5 – 6.7 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MES is often used in buffered culture media for bacteria, yeast, and mammalian cells. It is toxic towards plants at high concentrations, but can be used in culture media at lower concentrations for plants. Due to low ionic mobility, MES is regarded as an excellent buffer for use in capillary electrochromatography. MES is often employed as a substitute for the highly toxic cacodylate, as well as a substitute for the non-zwitterionic buffers citrate and malate. MES complexes with some organotin(IV) molecules that are being explored as antitumor agents.

MES sodium salt can be mixed with MES free acid or MES free acid monohydrate to attain desired pH.

Product Specifications
MES Sodium Salt
sodium;2-morpholin-4-ylethanesulfonate;
Sodium;2-(N-Morpholino)ethanesulfonic acid

Reagent Grade

Formula: C₆H₁₂NO₄SNa

MW: 217.22 g/mol

Storage/Handling: Store in a dry and well-ventilated place.

PubChem Chemical ID: 23673676

Video: How to make a 0.5M MES Sodium Salt Buffer Solution