Description
GoldBio’s PBS (Phosphate Buffered Saline) Tablets provide a fast, consistent, and error-free way to prepare 1X PBS at pH 7.3–7.5.
Each pre-measured tablet dissolves to produce 100 mL of isotonic, non-toxic buffer containing 10 mM phosphate, 137 mM sodium chloride, and 2.7 mM potassium chloride, eliminating weighing errors, which saves you valuable time.
Ideal for immunoassays, protein purification, and cell washing, PBS tablets ensure consistent osmolarity and pH across your experiments.
Functional Highlights and Mechanism
Isotonic Buffer System
Maintains osmotic balance comparable to mammalian extracellular fluid.
Phosphate Buffering Capacity
Stabilizes pH around neutrality (7.3–7.5), helping to minimize protein denaturation and reduce cell stress under typical handling conditions.
Defined Ionic Strength
Supports protein structure, antibody binding efficiency, and cell viability.
Tablet-Based Delivery
Provides standardized buffer composition without manual salt weighing or titration.
Recommended Applications and Usage Notes
Recommended Applications
- ELISA wash buffer preparation
- Western blot and immunohistochemistry workflows
- D-Luciferin dissolution for in vivo imaging
- Flow cytometry sample preparation
- Protein purification equilibration and wash steps
- General laboratory washing and dilution buffer
Usage Tips and Considerations
- Use high-purity distilled or deionized water for preparation.
- Allow full dissolution before use to ensure uniform ionic strength.
- Sterile-filter prepared PBS for cell culture or in vivo applications.
- For D-Luciferin preparation, prepare fresh or aliquot and store appropriately.
- Avoid repeated exposure of tablets to moisture; reseal container promptly.
Common Research Applications
(Click each for more information)
Wash Buffer in Immunoassays (ELISA, Western Blot, IHC)
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Purpose: To remove unbound antibodies or reagents while maintaining physiological conditions.
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How It Works: PBS maintains isotonic osmolarity (~300 mOsm) and stable pH (7.3–7.5), preventing protein denaturation or cell damage during washing steps.
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Applications: ELISA plate washing, antibody incubation steps, blocking buffer preparation, immunohistochemistry.
Crowther, J. R. (2009). The ELISA Guidebook (2nd ed.). Humana Press.
Dilution and Suspension Buffer for Cells and Biomolecules
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Purpose: To dilute antibodies, enzymes, or resuspend cells without altering osmotic balance.
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How It Works: The 10 mM phosphate system buffers near physiological pH while 137 mM NaCl and 2.7 mM KCl maintain ionic strength comparable to extracellular fluid.
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Applications: Antibody dilution, protein storage, flow cytometry preparation, cell washing.
Freshney, R. I. (2021). Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications (8th ed.). Wiley-Blackwell.
Protein Purification and Chromatography Preparation
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Purpose: To maintain protein structure and solubility during purification and downstream analysis.
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How It Works: PBS stabilizes protein conformation by maintaining near-neutral pH and ionic strength compatible with many affinity purification systems.
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Applications: Antibody purification (e.g., Protein A/G affinity), enzyme assays, size-exclusion chromatography equilibration.
Scopes, R. K. (1994). Protein Purification: Principles and Practice.
Wingfield, P. T. (2015). Overview of the purification of recombinant proteins. Current Protocols in Protein Science, 80, 6.1.1–6.1.35.
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