MES Free Acid Monohydrate, Ultra Pure
MES monohydrate is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 5.5 – 6.7 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MES is often used in buffered culture media for bacteria, yeast, and mammalian cells. It is toxic towards plants at high concentrations, but can be used in culture media at lower concentrations for plants. Due to low ionic mobility, MES is regarded as an excellent buffer for use in capillary electrochromatography. MES is often employed as a substitute for the highly toxic cacodylate, as well as a substitute for the non-zwitterionic buffers citrate and malate. MES complexes with some organotin(IV) molecules that are being explored as antitumor agents.
MES free acid or MES free acid monohydrate can be mixed with MES sodium salt to acquire desired pH. Alternatively, MES free acid or MES free acid monohydrate can be titrated with sodium hydroxide to attain desired pH.
MES Free Acid, Monohydrate, Ultra Pure
2-(N-Morpholino)ethanesulfonic acid, Monohydrate
Formula: C6H13NO4S ∙ H2O
MW: 213.25 g/mol
Storage/Handling: Store in a dry and well-ventilated place.
PubChem Chemical ID: 16218417
Note: MES Hydrate (CAS# 1266615-59-1) and MES Monohydrate (CAS# 145224-94-8) differ only in the number of water molecules associated with the buffer in powder form. Regardless of the number of water molecules present, these buffers are identical when dissolved into an aqueous solution.
|Storage/Handling||Store desiccated at room temperature.|
This ultimate buffer selection guide is a fantastic graphical display, showing common buffer pH ranges and their associated pKa. Buffers on this chart are also categorized by family. It's perfect to print out for your lab.
Refer to this printable buffer-pH chart for common buffers, associated pKa and pH range. Information on this chart is displayed in vibrant colors, and makes a great print-out for your lab.
A user guide for the background, experimental use and technical discussion of GoldBio biological buffers.
The Biological Buffer Selection Guide includes catalog number, pH range, pKa, applications, stock solution protocol availability and comments for the most common biological buffers.