Bacitracin, USP Grade
Bacitracin is a cyclic polypeptide antibiotic isolated from Bacillus subtilis. It is made up of a variety of antimicrobial peptide fractions including A, A1, B, B1, B2, C, D, E, F, G, and X. This standard grade product is a mixture of these fractions, with bacitracin A exhibiting the most potent antimicrobial activity. Bacitracins B1 and B2 exhibit about 90% of the antimicrobial activity of bacitracin A when together. Other forms of bacitracin have not been studied extensively, but may have negligible antimicrobial properties. Bacitracin is freely soluble in aqueous solution.
Bacitracin targets the cell wall of gram-positive organisms such as Streptococcus pyogenes and Staphylococcus aureus. Bacitracin also functions as a useful tool to distinguish between group A and group B β-hemolytic Streptococcus species, which are susceptible and resistant, respectively.
Bacitracin functions through inhibiting phosphorylation of bactoprenol, a step essential to synthesize peptidoglycan. Consequently, cell lysis occurs. Bacitracin resistance is conferred through a BcrABC transporter that pumps bacitracin out of cells or through BacA which allows phosphorylation of bactoprenol via an alternative biosynthesis pathway.
Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.
Bacitracin, USP Grade
MW: 1,422.69 g/mol
Storage/Handling: Store at 2-8 °C.
PubChem Chemical ID: 24891503
|Store at 4°C.
Antibiotic Treatments of Microbe-Contaminated Cell Cultures
Procedure on the use of different antibiotics to eliminate fungal, bacterial and mycoplasma contamination of cell lines
Sample COA - B-070
Sample Certificate of Analysis - Bacitracin, USP Grade
COA for example only. Actual tests may differ.