Introduction to Interleukins
The interleukin family of cytokines is one of the largest and most studied of all the growth factors...
GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!
Recombinant Murine Interleukin 3
Mast-cell Growth Factor, MCGF
P-cell Stimulating Factor
Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor, P-cell stimulating factor, burst promoting activity, multicolony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor.
In addition to activated T cells, other cell types can also produce IL3, such as human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes. At the amino acid sequence level, mature human and murine IL3 share only 29% sequence identity. Consistent with this lack of homology, IL3 activity is highly species-specific and human IL3 does not show activity on murine cells.
Formulation: Sterile filtered white lyophilized powder. Purified and tested for use in cell culture.
Sequence: DTHRLTRTLN CSSIVKEIIG KLPEPELKTD DEGPSLRNKS FRRVNLSKFV ESQGEVDPED RYVIKSNLQK LNCCLPTSAN DSALPGVFIR DLDDFRKKLR FYMVHLNDLE TVLTSRPPQP ASGSVSPNRG TVEC
MW: ~14.8 kDa globular protein (134 amino acid)
Purity: >98% by SDS-PAGE and HPLC analyses
Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of >2.0×107 IU/mg.
Accession Number: P01586
MGI ID: 96552
Source: Escherichia coli
Storage/Handling: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage. The reconstituted sample can be apportioned into working aliquots and stored at -80 °C for up to 6 months. Avoid repeated freeze/thaw cycles.
Reconstitution: The sample should be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a siliconized tube using PBS that contains a 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C. Stock solutions should be aliquoted and stored at -80°C. Further dilutions should be made in appropriate buffered solutions containing BSA or serum.
Additional resources for this product may be found at:
NOT FOR HUMAN USE!
|Storage/Handling||Store desiccated at -20°C.|
BaF3 cell culture and Mitogenic assay for FGFR expressing BAF3 cells: by Dave Ornitz, Ornitz Lab. Washington University, St.Louis, MO
Colony Forming Assay from M. Fero: Fero Lab, Fred Hutchinson Cancer Research Center, Seattle, WA
Informational resource handbook for various growth factors; their background, utilization and their receptor information.
MuIL3 Product Description, Amino Acid Sequence, Reconstitution and Storage Conditions