GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!
Recombinant Human Fibroblast Growth Factor 10
Keratinocyte Growth Factor 2
FGF10/KGF2 is a member of the Fibroblast Growth Factor family of proteins. KGF2 cDNA encodes a 208 amino acid residue protein with a hydrophobic amino-terminal signal peptide. Human KGF2 shares 92% and 95% amino acid sequence identity with mouse and rat KGF2, respectively. In the FGF family, KGF2 is most closely related to FGF7 (KGF1), FGF3 and FGF22 (see additional information). The expression of KGF2 transcript has been shown to be most abundant in the embryo and adult lung. Recombinant KGF2 has been shown to be mitogenic for epithelial and epidermal cells but not fibroblasts. KGF2 may play unique roles in brain and lung development, wound healing and limb bud formation.
Formulation: Sterile filtered white lyophilized powder. Purified and tested for use in cell culture.
Sequence: LGQDMVSPEA TNSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS
MW: ~19.1 kDa (169 amino acids)
Purity: >96% by SDS-PAGE and HPLC analyses
Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.
Accession Number: O15520
HGNC ID: 3666
Source: Escherichia coli
Storage/Handling: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage. The reconstituted sample can be apportioned into working aliquots and stored at -80 °C for up to 6 months. Avoid repeated freeze/thaw cycles.
Reconstitution: The sample should be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a siliconized tube using PBS that contains a 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C. Stock solutions should be aliquoted and stored at -80°C. Further dilutions should be made in appropriate buffered solutions containing BSA or serum and 1 µg/ml heparin.
Additional resources for this product may be found at:
NOT FOR HUMAN USE!
|Storage/Handling||Store desiccated at -20°C.|
Relative mitogenic activity of FGFs against FGF Receptors compared to FGF1 (aFGF).
HuFGF10 Product Description, Amino Acid Sequence, Reconstitution and Storage Conditions