EGF, Human

GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!

Recombinant Human Epidermal Growth Factor
EGF
HMGF (Human Milk Growth Factor)
PGF (Prostatic Growth Factor)

EGF (Epidermal Growth Factor) is a family of growth factors that are derived from membrane-anchored precursors. The family is characterized by the presence of at least one “EGF-like domain” (characterized by the presence of three disulfide bonds, formed from 6 conserved cysteine residues) in their extracellular domain. EGF was originally discovered as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition and stunting of growth when injected into newborn mice. Mature human EGF shares 70% amino acid sequence identity with mature mouse and rat EGF. Human EGF was isolated from urine based on its inhibitory effect on gastric secretion and is sometimes called urogastrone.

Formulation: Sterile filtered white lyophilized powder. Purified and tested for use in cell culture.

Sequence: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

MW: ~6.2 kDa (53 amino acid)

Purity: >98% by SDS-PAGE and HPLC analyses

Biological activity: Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine BALB/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of >1.0×10⁶ IU/mg.

Accession Number: P01133

HGNC ID: 3229

Source: Escherichia coli

Storage/Handling: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage. The reconstituted sample can be apportioned into working aliquots and stored at -80 °C for up to 6 months. Avoid repeated freeze/thaw cycles.

Reconstitution: The sample should be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a siliconized tube using PBS that contains a 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C. Stock solutions should be aliquoted and stored at -80°C. Further dilutions should be made in appropriate buffered solutions.

Additional resources for this product may be found at:

OMIM

NOT FOR HUMAN USE!