Tetracycline Hydrochloride

Tetracycline is a broad-spectrum antibiotic targeting gram-negative and gram-positive bacteria. It also displays toxicity against certain eukaryotic cells. Tetracycline is used in multidrug resistance studies and in cell culture applications as a selective agent. Additionally, it promotes expression of the P450 proteins.

Tetracyclines are antibiotics made up of a four-ring system. They bind the 30s ribosomal subunit, preventing the aminoacyl-tRNA from attaching to the A site. Consequently, protein synthesis is inhibited. Resistance to tetracycline arises from loss of cell wall permeability and the tetR gene.

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.

TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS.

Product Specifications
Tetracycline Hydrochloride

MOLECULAR BIOLOGY GRADE

Formula: C ₂₂H₂₄N₂O₈HCl

MW: 480.9 g/mol

Storage/Handling: Store desiccated at -20°C, and protect from light. Soluble in water.

PubChem Chemical ID: 5359252