Description
Nourseothricin is a high-purity, broad-spectrum antibiotic widely used as a dominant selectable marker in molecular biology and genetic engineering. It provides efficient selection across bacterial, yeast, fungal, and plant systems through its compatibility with the SAT1 resistance gene.
Researchers choose nourseothricin for its orthogonal selection properties, low working concentrations, and strong performance in CRISPR-Cas9 editing, plasmid maintenance, and marker recycling. With excellent solubility and storage stability, this reagent is ideal for scientists developing multi-gene systems or working in dual-host vectors.
GoldBio delivers consistent, publication-proven quality, making it a trusted choice for advanced genetic transformation applications.
Although nourseothricin sulfate is a streptothricin antibiotic, it functions via a mechanism similar to that of the aminoglycoside antibiotics. It induces miscoding of mRNA and leads to inhibition of protein synthesis.
GoldBio nourseothricin is a mixture of four streptothricin compounds: D and F (>85%) as well as C and E (<15%).
TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS AT GOLD BIOTECHNOLOGY LABS.
Common Research Applications
(Click each for more information)
Selective Marker in Bacterial Genetics (nat1/SAT1 Cassette Systems)
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Purpose: Nourseothricin is used as a selective agent in E. coli and other bacterial systems carrying the SAT1 streptothricin acetyltransferase resistance gene.
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How It Works: Nourseothricin inhibits prokaryotic translation by targeting the ribosome, while SAT1 confers resistance by enzymatically acetylating and inactivating the drug.
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Applications: Bacterial gene knockout workflows, plasmid selection, and chromosomal integrations.
Goldstein, A. L., & McCusker, J. H. (1999). Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae. Yeast, 15(14), 1541–1553.
Dual Antibiotic Selection in Yeast and Fungal Systems
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Purpose: Enables combinatorial selection in yeast and filamentous fungi alongside other antibiotics such as hygromycin or G418.
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How It Works: Expression of the SAT1 gene provides resistance to nourseothricin, allowing it to be paired with other resistance markers for multiple gene disruptions, co-transformations, or marker recycling.
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Applications: Gene disruption, CRISPR selection, and sequential editing in Candida albicans, Saccharomyces cerevisiae, and other model fungi.
Morschhäuser, J., Michel, S., & Hacker, J. (2004). The SAT1 flipper, an optimized tool for gene disruption in Candida albicans. Gene, 341, 119–127.
Marker for CRISPR-Cas9 Genome Editing in Yeast and Fungi
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Purpose: Nourseothricin is frequently used in CRISPR workflows that require orthogonal or recyclable selection markers.
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How It Works: Its unique resistance profile makes it useful for combining with other markers such as KanR or HygR in iterative editing or multi-gene manipulation workflows.
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Applications: CRISPR-Cas9 mediated gene deletions, insertions, and conditional editing in S. cerevisiae, Candida, and filamentous fungi.
Generoso, W. C., Gottardi, M., Oreb, M., & Boles, E. (2016). Simplified CRISPR-Cas genome editing for Saccharomyces cerevisiae. Journal of Microbiological Methods, 127, 203–205.
Selectable Marker in Transgenic Plant Cells
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Purpose: Offers an alternative selection system for genetically modified plant cells, especially where traditional antibiotics are ineffective or less desirable.
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How It Works: The sat3 gene, under a plant-compatible promoter such as CaMV 35S, confers resistance to nourseothricin through acetylation, allowing selective survival of transformed plant cells.
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Applications: Transformation of tobacco, Arabidopsis, and other model plants for regenerating stable transgenic lines.
Jelenska, J., Tempé, J., & Brevet, J. (2000). Streptothricin resistance as a novel selectable marker for transgenic plant cells. Plant Cell Reports, 19, 298–302.
Plasmid Maintenance in Shuttle Vectors for Bacteria and Yeast
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Purpose: Ensures stable maintenance and selection of plasmids containing SAT1 resistance cassettes across bacterial and fungal hosts.
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How It Works: Nourseothricin allows positive selection in both prokaryotic and eukaryotic systems without interference from commonly used antibiotics.
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Applications: Shuttle vectors for molecular cloning, episomal expression, and dual-host systems involving E. coli and S. cerevisiae.
Wach, A., Brachat, A., Pöhlmann, R., & Philippsen, P. (1994). New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast, 10(13), 1793–1808.
Benefits:
- Orthogonal Selection Marker: Nourseothricin provides an alternative to common antibiotics like G418, hygromycin, and puromycin, ideal for multigene constructs and CRISPR editing.
- Broad Host Range: Effective in prokaryotic and eukaryotic systems including E. coli, yeast, fungi, and plants.
- Efficient Selection: High specificity and potency enable low working concentrations, reducing background growth.
- Complement to Genetic Engineering Systems: Widely compatible with SAT1-based resistance plasmids and cassettes.
- Stability and Shelf-Life: Powder format with excellent stability under standard storage for extended usability.
Storage/Handling:
Store desiccated at 4°C.
PubChem Chemical ID:
Streptothricin D: 202550, Streptothricin F: 197034, Streptothricin E: 160718, Streptothricin C: 165157
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