This is the hub page for for CRISPR, PCR and DNA Gel Electrophoresis.
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Find out more about how our reliable enzymes, products, protocols, troubleshooting tips and resources can help you study and manipulate nucleic acids and genetic material. We offer high-quality PCR reagents for your DNA amplification reaction: dNTPs, PCR additives, and Taq, High Fidelity and Hot Start DNA Polymerases. Also, our InstantAmp™ Plant Direct PCR Kit is designed to be the simplest and best method to efficiently amplify DNA fragments from plant tissues. In addition, we provide a wide range of products including reverse transcriptase (RT) and reverse transcription-polymerase chain reaction (RT-PCR) Kits that allow cDNA generation and detection of RNA. We also carry efficient ligases, T7 exonuclease and T7 endonuclease I that are ideal for nucleic acid manipulation.
Using quantitative PCR (qPCR), also known as real time quantitative PCR (RT-qPCR) and quantitative real time PCR (qRT-PCR), we can analyze the quantity (copy number) of a target DNA sequence as it is replicated, in real time. Here, you can find GoldBio products ideal for robust amplification and accurate gene expression quantification. Our EvaGreen® dye, a great alternative to SYBR® Green I, Goof-Proof™ qPCR Master Mix, and RT-qPCR Kit are optimal for use in your DNA melt curve analysis and real time-PCR (RT-PCR) reactions.
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This molecular copying operates through cycles of thermal reactions enabled by an assembly of biochemical reagents, and amplifies a few copies of DNA to millions. Amplified DNA can then be analyzed qualitatively (for its presence), quantitatively (by amount) or sequentially (for its genetic code), and used in downstream molecular biology applications. 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CRISPR Products & Resources
The CRISPR/Cas gene editing system is a breakthrough technology currently being used in many labs to modify genomes. Learn about or trusted CRISPR products and supporting tools here. We offer Cas9 nucleases, a T7 endonuclease, Pfu DNA Polymerases as well as comprehensive protocols to perform CRISPR-mediated genome manipulation. We also offer informative articles discussing the uses–transcriptional regulation, epigenetic modification, nucleic acid modification, RNA editing and ssDNA detection–and downstream applications of CRISPR.
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DNA Gel Electrophoresis Products & Resources
Gel electrophoresis is a technique commonly used to separate DNA fragments by size. We have developed a collection of high purity agarose products–Agarose LE, Low Melt Agarose and High Resolution Agarose–to help you generate an efficient agarose gel for accurate separation of your nucleic acid samples. In addition, you can find a variety of DNA ladders and loading dyes here, including our ultra-sensitive fluorescent nucleic acid dye 6x GelRed™, which is a safer alternative to ethidium bromide (EtBr).
Our ladder comparison charts, comprehensive protocols and video demonstrations also found below will guide you through the DNA gel electrophoresis process.
InstantAmp™ Viral Solution for Covid-19
Product Description
GoldBio’s InstantAmp™ Viral Solution for Covid-19 can be utilized for saliva/swab samples resulting in viral/cell lysis, and significantly simplifying this process. This solution streamlines viral specimen collection and viral RNA preparation processes including sample collection, transport, maintenance, viral/cell lysis, and viral RNA stabilization. After using the solution, the lysed viral RNA may be directly used in RT-qPCR, RT-isothermal DNA amplification, or any direct diagnosis kits for Covid-19 testing, without the need of an RNA extraction step.
InstantAmp™ Viral/Cell Solution for Covid-19 Testing can be used to detect SARS-CoV-2 in any type of respiratory specimens such as swabs, saliva, sputum, nasopharyngeal, oropharyngeal aspirates, washes or/and tracheal aspirates.
This solution allows for efficient viral sample collection, processing, and SARS-CoV-2 viral RNA preparation in a single tube.
For research use only. Not to be used for diagnostic purposes. Please contact us prior to purchase for more information on recommendations for testing and applications.
Innovation
Part of what also makes InstantAmp™ Viral Solution for Covid-19 Testing an innovative product is the fact that it contains very low concentration of less toxic chemicals different from others. The CDC currently recommends viral lysis buffers and/or RNA extraction solutions from companies whose products are not only expensive, but also contain a high concentration of chemicals known to be toxic to humans and the environment. Our solution makes Covid-19 testing simpler, faster, safer and more affordable!
Benefits
- No RNA extraction is needed
- Safe for sample transport and maintenance
- Speeds up the Covid-19 testing process
- Compatible with all type of viral specimens
- Compatible with different detection technologies
- Increased efficiency, specificity, and sensitivity
- Low toxicity to humans/environment
Product Includes
- InstantAmp™ Viral Solution
Quality Control
Functionally tested in RT-qPCR based on CDC recommended probe for activity, processivity, efficiency and sensitivity.
Storage/Handling: Store at room temperature.
References
- https://www.fda.gov/media/134922/download
- Blow JA, Dohm DJ, Negley DL, Mores CN (2004) Virus inactivation by nucleic acid extraction reagents. J Virol Methods 119:195-198.
For research use only. Not to be used for diagnostic purposes. Please contact us prior to purchase for more information on recommendations for testing and applications.
Technical Documentation
P
Protocol
O
Other
C
Certificate of Analysis
S
Safety Data Sheet
