Protein Ladders: Prestained Versus Unstained
There are two categories of commercially available protein ladders, unstained protein ladders and pr...
Ponceau S is the sodium salt of a diazo dye used as a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF.It is often used in the staining of polypeptides to be later blot-sequenced because it does not adversely affect the sequencing of the blotted peptides.The stain is also reversible with water washes, preferable for immunological detection, and can even be completely removed.Ponceau S is a negatively charged stain which binds to positively charged amino acids as well as non-polar regions of proteins. The limit of detection for Ponceau S is ~250 ng of protein.
GoldBio’s Ponceau S solution is provided ready to use.
Ponceau S in 5% acetic acid
1 mg/ml (0.1%) Pre-made Solution
MOLECULAR BIOLOGY GRADE
MW: 760.57 g/mol
Storage/Handling: Store at 4°C.
PubChem Chemical ID: 6364532
|Grade||MOLECULAR BIOLOGY GRADE|