This is the hub page for for CRISPR, PCR and DNA Gel Electrophoresis.
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Find out more about how our reliable enzymes, products, protocols, troubleshooting tips and resources can help you study and manipulate nucleic acids and genetic material. We offer high-quality PCR reagents for your DNA amplification reaction: dNTPs, PCR additives, and Taq, High Fidelity and Hot Start DNA Polymerases. Also, our InstantAmp™ Plant Direct PCR Kit is designed to be the simplest and best method to efficiently amplify DNA fragments from plant tissues. In addition, we provide a wide range of products including reverse transcriptase (RT) and reverse transcription-polymerase chain reaction (RT-PCR) Kits that allow cDNA generation and detection of RNA. We also carry efficient ligases, T7 exonuclease and T7 endonuclease I that are ideal for nucleic acid manipulation.
Using quantitative PCR (qPCR), also known as real time quantitative PCR (RT-qPCR) and quantitative real time PCR (qRT-PCR), we can analyze the quantity (copy number) of a target DNA sequence as it is replicated, in real time. Here, you can find GoldBio products ideal for robust amplification and accurate gene expression quantification. Our EvaGreen® dye, a great alternative to SYBR® Green I, Goof-Proof™ qPCR Master Mix, and RT-qPCR Kit are optimal for use in your DNA melt curve analysis and real time-PCR (RT-PCR) reactions.
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This molecular copying operates through cycles of thermal reactions enabled by an assembly of biochemical reagents, and amplifies a few copies of DNA to millions. Amplified DNA can then be analyzed qualitatively (for its presence), quantitatively (by amount) or sequentially (for its genetic code), and used in downstream molecular biology applications. 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CRISPR Products & Resources
The CRISPR/Cas gene editing system is a breakthrough technology currently being used in many labs to modify genomes. Learn about or trusted CRISPR products and supporting tools here. We offer Cas9 nucleases, a T7 endonuclease, Pfu DNA Polymerases as well as comprehensive protocols to perform CRISPR-mediated genome manipulation. We also offer informative articles discussing the uses–transcriptional regulation, epigenetic modification, nucleic acid modification, RNA editing and ssDNA detection–and downstream applications of CRISPR.
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DNA Gel Electrophoresis Products & Resources
Gel electrophoresis is a technique commonly used to separate DNA fragments by size. We have developed a collection of high purity agarose products–Agarose LE, Low Melt Agarose and High Resolution Agarose–to help you generate an efficient agarose gel for accurate separation of your nucleic acid samples. In addition, you can find a variety of DNA ladders and loading dyes here, including our ultra-sensitive fluorescent nucleic acid dye 6x GelRed™, which is a safer alternative to ethidium bromide (EtBr).
Our ladder comparison charts, comprehensive protocols and video demonstrations also found below will guide you through the DNA gel electrophoresis process.
Hub page housing all our competent cells, grouped.
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GoldBio offers an extensive collection of efficient chemically competent and electrocompetent cells with high transformation efficiencies to match your specific downstream applications: general cloning, protein expression, library construction, and many more.
- GoldBio's wide range of chemically competent and electrocompetent cells are optimized to provide lot-to-lot consistency and up to 10X higher transformation efficiency.
- Our enhanced recovery medium (SOC) formulation accompanies all of GoldBio's competent cells.
- We carry multiple Endonuclease I-deficient (endA1) competent bacterial cell strains to suit your cloning needs and ensure your transformations result in high quality and yield of plasmid DNA.
- Choose from E. coli strains with different selectable markers and screening options including the classic blue/white selection.
- GoldBio cells are ideal for distinct downstream applications from routine cloning/subcloning and protein expression to the more complex library construction, and cloning of difficult targets.
- Our electrocompetent Agrobacterium cells contain an efficient T-DNA binary system for genetic material transfer into the host plant cells.
- GoldBio's amber suppressor (SupE) and non-amber suppressor (MC1061F\’) strains are ideal for phage display library screening.
CHOOSE THE BEST COMPETENT CELLS FOR YOUR APPLICATION!
Our DH5-alpha, BL21, BL21(DE3), DH10B, DH10B-Pro, DL39(DE3), Agrobacterium, L. lactis, SS320 and TG1 Phage Display cells are available in multiple formats to provide you with flexibility when planning your experiments. GoldBio also provides easy-to-follow protocols, FAQs, protocol videos and selection guides to ensure success of your transformations!
GoldBio Competent Cells Consistently Exhibit Higher Transformation Efficiencies
DH10B chemically competent E. coli cells and DH10B Electrocompetent E. coli cells exhibit higher transformation efficiency than competitors. Each transformation with DH10B chemically competent E. coli cells was performed using 1 ml of final volume including recovery media, plating 50 μl after a 10 fold-dilution (left). For electrocompetent cell comparison, DH10B Electrocompetent E. coli cells were electroporated using a 1 ml of final volume including recovery media, plating 50 μl after 100-fold dilution (right).
BROWSE OUR TRANSFORMATION AND COMPETENT CELL RESOURCES
Your time in the lab is valuable! In this section you will find GoldBio's transformation tools designed to help support and simplify your competent cell transformations. You can access specific chemical transformation or electroporation protocols for each of our competent cell lines, antibiotic information, colony screening protocols, instructional blog articles, detailed guides containing competent cells characteristics and downstream applications, FAQs, electroporation troubleshooting tips and video protocols.
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Electroporation is a technique commonly used in transformation assays to introduce DNA into an electrocompetent cell. During electroporation a transient current or electrical pulse is applied to cells that have been suspended in a solution. The current momentarily increases the permeability of the cell's membrane, creating holes (a pathway) that allow DNA (plasmids) to pass through and enter the cell. Generally, electroporation can be more effective than chemical transformation.
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" } } } [2]=> array(7) { ["id"]=> int(21) ["name"]=> string(3) "FAQ" ["slug"]=> string(3) "faq" ["description"]=> NULL ["show_in_picker"]=> int(1) ["deleted_on"]=> NULL ["fields"]=> array(2) { [0]=> array(22) { ["id"]=> int(75) ["parent_id"]=> int(0) ["field_group_id"]=> int(21) ["name"]=> string(8) "Question" ["slug"]=> string(8) "question" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(9) "TextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(1) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> string(47) "How do I prevent arcing during electroporation?" } [1]=> array(22) { ["id"]=> int(76) ["parent_id"]=> int(0) ["field_group_id"]=> int(21) ["name"]=> string(6) "Answer" ["slug"]=> string(6) "answer" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(13) "RichTextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(2) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> string(372) "Arcing during electroporation can be avoided by making sure there are no air bubbles in the cell/DNA solution prior to electroporation. Also, desalt your DNA since the presence of conductive ions in the solution will cause arcing and electroporation failure. In addition, ensure that your equipment, including the cuvette is properly cleaned and in good condition.
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Product Description
i7® High-Fidelity DNA Polymerase 2X Master Mix is ready to use premix which contains i7 high-fidelity DNA Polymerase, dNTPs, MgCl2, PCR enhancers and stabilizers with optimized proprietary reaction buffer. i7® High-Fidelity DNA Polymerase is a genetically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This enzyme has the high-fidelity, sensitivity and processivity with an error rate ~2.8 × 102-fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes in the marketplace. This 2X master mix product is supplied with 5X GC enhancer that enables efficient amplification of GC rich templates up to 84%.
Applications
- Long and difficult template DNA amplification
- Cloning
- High-fidelity PCR
- Efficient for amplifying high GC content template DNA with GC enhancer.
Product Includes
- i7® High-Fidelity DNA Polymerase 2x master mix
- 5X GC Enhancer
Activity Data
i7® high-fidelity 2x master mix has been tested with difficult templates for PCR amplification. Typical PCR results are shown below:
Storage Buffer
50mM Tris-HCl, 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol, pH 7.5 @ 25°C
Heat Inactivation: No
Storage/Handling: Store at -20°C.
Product may be shipped on blue ice.
Technical Documentation
P
Protocol
O
Other
C
Certificate of Analysis
S
Safety Data Sheet
