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Troubleshooting PCR Part Three: Solutions for Weak Bands and Smearing
When doing PCR, you can encounter all sorts of issues, which is why we have been doing a series on...
dNTP Mix
Product Description
GoldBio’s 2’-Deoxynucleoside 5’-triphosphate mix is an aqueous solution containing dATP, dCTP, dGTP and dTTP, each at a final concentration of 10mM. This mixture has been tested for performance in a wide size-range of PCR templates with Taq and Pfu DNA polymerases.
The dNTP solution is ideal for PCR, cDNA synthesis, DNA sequencing and labeling procedures, and it has been performance tested with Q-PCR and long PCR (30 kb).
Product Specifications
dNTP Mix
Certified free of nucleases (DNase, RNase) and phosphatases
Concentration: 10mM each (dATP, dCTP, dGTP and dTTP)
Storage/Handling: Store at -20°C.
Avoid freeze/thaw cycles or exposure to frequent temperature changes.
dNTPs Quick Answers:
How much dNTPs are used for PCR?
What are dNTPs?
dNTPs are the building blocks for DNA synthesis composed of a nitrogenous base that is bound to a deoxyribose with a triphosphate. The four dNTPs used in DNA synthesis are abbreviated based on their nitrogenous based: dATP represents adenine, dCTP represents cytosine, dGTP represents guanine, and dTTP represents thymine.
What does dNTP stand for?
dNTPs stand for deoxyribonucleotide triphosphate, and uses each of the four bases of DNA: dATP for adenine, dCTP for cytosine, dGTP for guanine, and dTTP for thymine.
How much dNTPs are used for PCR?
Most protocols suggest using a final concentration of 200µM for 10mM dNTPs.
|
Reaction Volume |
dNTP Volume |
Final Concentration |
|
25 µl |
0.5 µl |
200 µM |
|
50 µl |
1 µl |
200 µM |
The role of dNTPs in PCR?
In PCR, dNTPs function as the building blocks for DNA amplification. Here, the four dNTPS (dATP, dCTP, dGTP and dTTP) are added by Taq polymeras to a template strand of DNA at the corresponding base. Another way of looking at it is the role of dNTPs are to function as the necessary nucleotides to build a growing strand of DNA. The addition of dNTPs along a template strand of DNA in PCR build the second, complementary strand of DNA.
How to store dNTPs?
Store dNTPs at -20°C. With dNTPs, it is very important to avoid freeze/thaw cycles and frequent temperature changes.
How are dNTPs shipped?
To ensure the stability and efficiency of dNTPs, GoldBio ships dNTPs next day on blue ice.
Product Specifications
| Catalog ID | D-900 |
|---|---|
| Storage/Handling | Store at -20°C. |
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