• object(anvilstl\commercio\models\Category)#394 (15) { ["featured_image_urls"]=> array(3) { ["small"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/Dna-Protien-Electrophoresis.jpg" ["medium"]=> string(97) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/Dna-Protien-Electrophoresis.jpg" ["large"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/Dna-Protien-Electrophoresis.jpg" } ["featured_image"]=> array(3) { ["small"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/Dna-Protien-Electrophoresis.jpg" ["medium"]=> string(97) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/Dna-Protien-Electrophoresis.jpg" ["large"]=> string(96) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/Dna-Protien-Electrophoresis.jpg" } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(198) ["root"]=> int(393) ["lft"]=> int(40) ["rgt"]=> int(45) ["level"]=> int(1) ["name"]=> string(35) "DNA & Protein Electrophoresis (old)" ["url_name"]=> string(27) "dna-protein-electrophoresis" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(439) "

    Whether you need to analyze PCR results or produce a large quantity of purified proteins, GoldBio offers the tools necessary for DNA and protein techniques. GoldBio’s inventory includes a quality selection of agaroses, ready-to-use loading dyes and buffers, DNA and protein ladders, Coomassie stains, and more. With GoldBio’s low prices and first-rate products, we have everything needed to put your research into action.

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    Whether you need to analyze PCR results or produce a large quantity of purified proteins, GoldBio offers the tools necessary for DNA and protein techniques. GoldBio’s inventory includes a quality selection of agaroses, ready-to-use loading dyes and buffers, DNA and protein ladders, Coomassie stains, and more. With GoldBio’s low prices and first-rate products, we have everything needed to put your research into action.

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    Gold Biotechnology offers a variety of lysis buffers fit for bacterial, animal, plant or yeast cultures, as well as IPTG, which is also available in an easy to use EZ Pak, and 5-Fluoroorotic Acid, ideal for yeast selection. These tools are fundamental for induction, selection and protein purification. GoldBio carries quality products at a fraction of the price of other suppliers, allowing you to focus your research funds on the things that really matter.

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    Gold Biotechnology offers a variety of lysis buffers fit for bacterial, animal, plant or yeast cultures, as well as IPTG, which is also available in an easy to use EZ Pak, and 5-Fluoroorotic Acid, ideal for yeast selection. These tools are fundamental for induction, selection and protein purification. GoldBio carries quality products at a fraction of the price of other suppliers, allowing you to focus your research funds on the things that really matter.

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    Polymerase chain reaction (PCR), clustered regularly interspaced short palindromic repeats (CRISPR) and DNA gel electrophoresis are powerful molecular biology techniques. Here at GoldBio, we understand the reagents and enzymes you need to perform these techniques successfully in your lab must be of the highest quality. Therefore, we have developed a collection of superior products including dNTPs, DNA polymerases, reverse transcriptases, ligases, master mixes, PCR and quantitative PCR kits, protocols and tools to help you achieve your research goals.

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    PCR Products & Resources

    Find out more about how our reliable enzymes, products, protocols, troubleshooting tips and resources can help you study and manipulate nucleic acids and genetic material. We offer high-quality PCR reagents for your DNA amplification reaction: dNTPs, PCR additives, and Taq, High Fidelity and Hot Start DNA Polymerases. Also, our InstantAmp™ Plant Direct PCR Kit is designed to be the simplest and best method to efficiently amplify DNA fragments from plant tissues. In addition, we provide a wide range of products including reverse transcriptase (RT) and reverse transcription-polymerase chain reaction (RT-PCR) Kits that allow cDNA generation and detection of RNA. We also carry efficient ligases, T7 exonuclease and T7 endonuclease I that are ideal for nucleic acid manipulation.

    Using quantitative PCR (qPCR), also known as real time quantitative PCR (RT-qPCR) and quantitative real time PCR (qRT-PCR), we can analyze the quantity (copy number) of a target DNA sequence as it is replicated, in real time. Here, you can find GoldBio products ideal for robust amplification and accurate gene expression quantification. Our EvaGreen® dye, a great alternative to SYBR® Green I, Goof-Proof™ qPCR Master Mix, and RT-qPCR Kit are optimal for use in your DNA melt curve analysis and real time-PCR (RT-PCR) reactions.

    " } } } [1]=> array(7) { ["id"]=> int(18) ["name"]=> string(4) "Card" ["slug"]=> string(4) "card" ["description"]=> NULL ["show_in_picker"]=> int(1) ["deleted_on"]=> NULL ["fields"]=> array(5) { [0]=> array(22) { ["id"]=> int(69) ["parent_id"]=> int(0) ["field_group_id"]=> int(18) ["name"]=> string(5) "Title" ["slug"]=> string(5) "title" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(9) "TextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(1) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> string(12) "PCR Overview" } [1]=> array(22) { ["id"]=> int(70) ["parent_id"]=> int(0) ["field_group_id"]=> int(18) ["name"]=> string(11) "Description" ["slug"]=> string(11) "description" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(13) "TextAreaField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(2) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> string(533) "PCR involves the use of DNA polymerase to synthesize complementary strands of DNA from a template effectively doubling the original sample with every cycle of the PCR reaction. This molecular copying operates through cycles of thermal reactions enabled by an assembly of biochemical reagents, and amplifies a few copies of DNA to millions. Amplified DNA can then be analyzed qualitatively (for its presence), quantitatively (by amount) or sequentially (for its genetic code), and used in downstream molecular biology applications. 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    CRISPR Products & Resources

    The CRISPR/Cas gene editing system is a breakthrough technology currently being used in many labs to modify genomes. Learn about or trusted CRISPR products and supporting tools here. We offer Cas9 nucleases, a T7 endonuclease, Pfu DNA Polymerases as well as comprehensive protocols to perform CRISPR-mediated genome manipulation. We also offer informative articles discussing the uses–transcriptional regulation, epigenetic modification, nucleic acid modification, RNA editing and ssDNA detection–and downstream applications of CRISPR.

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    DNA Gel Electrophoresis Products & Resources

    Gel electrophoresis is a technique commonly used to separate DNA fragments by size. We have developed a collection of high purity agarose products–Agarose LE, Low Melt Agarose and High Resolution Agarose–to help you generate an efficient agarose gel for accurate separation of your nucleic acid samples. In addition, you can find a variety of DNA ladders and loading dyes here, including our ultra-sensitive fluorescent nucleic acid dye 6x GelRed™, which is a safer alternative to ethidium bromide (EtBr).

    Our ladder comparison charts, comprehensive protocols and video demonstrations also found below will guide you through the DNA gel electrophoresis process.


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    • Hot Start Taq 2x Master Mix - 50 µL reaction

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    Product Description

    Hot start Taq DNA Polymerase 2x master mix is ready to use premix which contains hot start Taq DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer that has been optimized for routine PCR applications. Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This product is supplied with an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.

    Product Includes

    • Hot Start Taq 2x master mix
    • 5x GC Enhancer

    Taq Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)

    Taq Concentration (1x): 0.1 units/μl
    One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72ºC.

    Source: E. coli strain expressing a Taq DNA Polymerase gene from Thermus aquaticus YT-1

    Storage/Handling: Store at -20°C.
    Product may be shipped on blue ice.

    Product Specifications

    Catalog ID T-512
    Storage/Handling Store at -20°C.

    Technical Documentation

    P Protocol
    O Other
    C Certificate of Analysis
    S Safety Data Sheet
    P
    PCR Protocol with Hot Start Taq 2x Master Mix

    Protocol for PCR reaction using Hot Start Taq 2X Master Mix.

    S
    SDS (MSDS) - Hot Start Taq 2x Master Mix

    Safety Data Sheet

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