Sisomicin is a broad-spectrum aminoglycoside antibiotic isolated from Micromonospora inyoensis. It is related to gentamicin and targets gram-negative and gram-positive bacteria such as Klebsiella species, E. coli, P. aeruginosa, Enterobacter species, and Proteus species. It has been used in disk and tube dilution sensitivity assays.
Aminoglycoside antibiotics are composed of amino groups attached to glycosides. They bind the 30s ribosomal subunit, causing misreading of the mRNA sequence and inhibition of translocation. Consequently, protein synthesis is inhibited.
Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.
Formula: 2 C19H37N5O7 ∙ 5 H2SO4
MW: 692.72 g/mol
Storage/Handling: Store at 4°C.
PubChem CID: 20055515
|MOLECULAR BIOLOGY GRADE
|Store at 4°C.