Penicillin G Sodium Salt, USP Grade
Penicillin G is a β-lactam antibiotic produced by the Penicillium fungus. It has shown efficacy against a variety of Streptococcus species as well as non-penicillinase producing Staphylococcus species.
Penicillins are a type of β-lactam antibiotic consisting of a four-membered β-lactam ring bound to a five-membered thiazolidine ring. This two-ring system causes distortion of the β-lactam amide bond, resulting in decreased resonance stabilization and increased reactivity. β-lactams inhibit the formation of peptidoglycan cross-links within bacterial cell walls by targeting penicillin-binding proteins or PBPs. Consequently, the bacterial cell wall becomes weak and cytolysis occurs. Resistance to β-lactam antibiotics occurs in the presence of cells containing plasmid encoded extended spectrum β-lactamases or ESBLs.
Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.
Penicillin G Sodium
M.W.: 356.37 g/mol
Storage/Handling: Store desiccated at -20°C.
PubChem CID 23668834
|Store desiccated at -20°C.
BRET (Bioluminescence Resonance Energy Transfer) Method
High Throughput Bioluminescence Resonance Energy Transfer (BRET) Method using Rluc and Coelenterazine by SW Gersting, et al. (2012)
Antibiotic Treatments of Microbe-Contaminated Cell Cultures
Procedure on the use of different antibiotics to eliminate fungal, bacterial and mycoplasma contamination of cell lines
Sample COA - P-304
Sample Certificate of Analysis - Penicillin G Sodium Salt
COA for example only. Actual tests may differ.