Troubleshooting PCR Part Two: What to Do When You Are Not Getting Bands
PCR (Polymerase Chain Reaction), which is so common to the lab, can be very frustrating. We unders...
Dimethylsulfoxide (DMSO) is a very polar organic compound that is useful in organic chemistry for solubilizing organic and inorganic compounds. DMSO is also used as a cryoprotectant for cell media, preventing the formation of ice crystals which would otherwise damage cells. DMSO can be used in PCR reactions to inhibit the self-complementation of DNA or PCR primers and increase amplification, especially for DNA sequences which have GC rich sequences.
GoldBio's DMSO is filter sterilized through a 0.2 μg filter. Sterile DMSO is ideal for working with tissue culture or cell culture in which there is a need for eliminating all potential sources of contamination.
MW: 78.13 g/mol
Storage/Handling: Store in a dry and well-ventilated place.
PubChem Chemical ID: 679
|Storage/Handling||Store at room temperature, protect from light.|
A protocol for the use of a methylumbelliferyl substrate for identifying yeast isolate enzymes by Bobey and Ederer (1981).
Procedure on the use of different antibiotics to eliminate fungal, bacterial and mycoplasma contamination of cell lines
Sample Certificate of Analysis - Sterile Filtered DMSO, Ultra Pure
COA for example only. Actual tests may differ.